Background: In Ayurvedic and Unani texts, many herbs and formulations are mentioned as complexion promoters having skin brightening and whitening effects that downregulate melanin synthesis. However, with the assistance of chromatographic techniques, further validation of scientific standardization is required. Objective: To validate individual herbs and formulations in the ancient literature, some scientific aspects are to be focused on, including standardization of herbs or herbal products consisting of the active compounds. Methods: Out of many herbs having complexion promoting properties, three herbs (Myristica fragrans, Hemidesmus indicus, and Inula racemosa) were selected for the study. In the present study, validated reverse phase (RP)-HPLC and high-performance TLC (HPTLC) methods were developed for standardization of the herbs. Results: It was observed that the quercetin present in M. fragrans was 0.62% (w/w) with a retention time (R_t) of 6.23 min, the ferulic acid present in H. indicus was 1.39% (w/w) with an R_t of 8.083 min, and the chlorogenic acid present in I. racemosa was 1.03% (w/w) with an R_t of 3.19 min. The HPTLC method showed 1.23% (w/w) of quercetin present in M. fragrans with a retardation factor (R_f) of 0.48, ferulic acid in H. indicus was 1.52% (w/w) with an R_f of 0.44, and chlorogenic acid was 1.09% (w/w) in I. racemosa with an R_f of 0.52. Conclusions: This specific and precise validated method can be useful for the quality evaluation and quantitative determination of the constituents in complexion-promoting herbs from Ayurveda and Unani systems of medicine. Highlights: The exploration of complexion promoters from Ayurveda and Unani; quality evaluation of complexion promoters herbs from Ayurveda and Unani; HPTLC and RP-HPLC analysis of herbal extracts used and their validation.

中文翻译：

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RP-HPLC和HPTLC方法分析阿育吠陀和Unani医药体系中用作肤色促进剂的精选草药。

背景：在阿育吠陀和乌纳尼教科书中，提到许多草药和配方作为肤色促进剂，具有增白和美白皮肤的作用，从而下调黑色素的合成。但是，借助于色谱技术，还需要进一步验证科学的标准化。目的：要验证古代文献中的各种草药和配方，应侧重于一些科学方面，包括草药或由活性化合物组成的草药产品的标准化。方法：将出具有肤色促进作用，三种药材许多草药（的肉豆蔻，Hemidesmus蜓和总状土木香选择）进行研究。在本研究中，开发了经过验证的反相（RP）-HPLC和高性能TLC（HPTLC）方法来标准化草药。结果：据观察，存在于槲皮素M.香为0.62％（重量/重量）与保留时间（R_吨的6.23分钟），阿魏酸存在于H.蜓为1.39％（重量/重量）与的R_吨的8.083分，和酸存在于绿原I.总状为1.03％（重量/重量）与的R_吨的3.19分钟。HPTLC方法显示存在于苦参中的槲皮素为1.23％（w / w），阻滞因子（R _f）为0.48，阿魏酸为H.蜓为1.52％（重量/重量）与的R _˚F 0.44，和绿原酸为1.09％（重量/重量）在一总状被R _˚F 0.52。结论：这种经过专门验证的精确方法可用于质量评估和定量测定阿育吠陀和Unani医药体系中促肤色的草药的成分。亮点：探索阿育吠陀和乌纳尼肤色促进剂；阿育吠陀和乌纳尼肤色促进剂草药的质量评估；所用草药提取物的HPTLC和RP-HPLC分析及其验证。