当前位置: X-MOL 学术J. Drug Target. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Gold nanoparticles potentiates N-acetylcysteine effects on neurochemicals alterations in rats after polymicrobial sepsis.
Journal of Drug Targeting ( IF 4.3 ) Pub Date : 2019-10-17 , DOI: 10.1080/1061186x.2019.1678168
Fabricia Petronilho 1 , Leonardo Tenfen 1 , Amanda Della Giustina 1 , Larissa Joaquim 1 , Michele Novochadlo 1 , Aloir Neri de Oliveira Junior 1 , Erick Bagio 1 , Mariana Pereira de Souza Goldim 1 , Raquel Jaconi de Carli 1 , Sandra Regina Santana de Aguiar Bonfante 1 , Kiuanne Lino Lobo Metzker 1 , Samara Muttini 2 , Thayná Marinho Dos Santos 2 , Mariana Pacheco de Oliveira 1 , Nicole Alessandra Engel 1 , Gislaine Tezza Rezin 1 , Luiz Alberto Kanis 2 , Tatiana Barichello 3, 4, 5
Affiliation  

Herein, we report the effect of gold nanoparticles (AuNP) and n-acetylcysteine (NAC) isolated or in association as important anti-inflammatory and antioxidant compounds on brain dysfunction in septic rats. Male Wistar rats after sham operation or caecal ligation and perforation (CLP) were treated with subcutaneously injection of AuNP (50 mg/kg) and/or NAC (20 mg/kg) or saline immediately and 12 h after surgery. Twenty-four hours after CLP, hippocampus and prefrontal cortex were obtained and assayed for myeloperoxidase (MPO) activity, cytokines, lipid peroxidation, protein carbonyls formation, mitochondrial respiratory chain, and CK activity. AuNP + NAC association decreased MPO activity and pro-inflammatory cytokines production, being more effective than NAC or AuNP isolated treatment. AuNP + NAC association and NAC isolated treatment decreased oxidative stress to lipids in both brain structures, while protein oxidation decreased only in the hippocampus of AuNP + NAC association-treated animals. Complex I activity was increased with AuNP + NAC association and NAC isolated in the hippocampus. Regarding CK activity, AuNP and AuNP + NAC association increased this marker in both brain structures after CLP. Our data provide the first experimental demonstration that AuNP and NAC association was able to reduce sepsis-induced brain dysfunction in rats by decreasing neuroinflammation, oxidative stress parameters, mitochondrial dysfunction and CK activity.

中文翻译:

金纳米粒子增强了 N-乙酰半胱氨酸对多微生物败血症后大鼠神经化学物质改变的影响。

在此,我们报告了分离或联合作为重要抗炎和抗氧化化合物的金纳米粒子 (AuNP) 和 n-乙酰半胱氨酸 (NAC) 对脓毒症大鼠脑功能障碍的影响。假手术或盲肠结扎穿孔 (CLP) 后的雄性 Wistar 大鼠在手术后立即和 12 小时皮下注射 AuNP (50 mg/kg) 和/或 NAC (20 mg/kg) 或生理盐水。CLP 后 24 小时,获取海马和前额叶皮层并测定髓过氧化物酶 (MPO) 活性、细胞因子、脂质过氧化、蛋白质羰基形成、线粒体呼吸链和 CK 活性。AuNP + NAC 联合降低 MPO 活性和促炎细胞因子的产生,比 NAC 或 AuNP 分离治疗更有效。AuNP + NAC 结合和 NAC 分离处理降低了两种大脑结构中脂质的氧化应激,而蛋白质氧化仅在 AuNP + NAC 结合处理的动物的海马中降低。复合物 I 活性随着 AuNP + NAC 结合和海马中分离的 NAC 而增加。关于 CK 活性,AuNP 和 AuNP + NAC 关联在 CLP 后增加了两种大脑结构中的这一标志物。我们的数据提供了第一个实验证明,AuNP 和 NAC 联合能够通过减少神经炎症、氧化应激参数、线粒体功能障碍和 CK 活性来减少大鼠败血症诱导的脑功能障碍。复合物 I 活性随着 AuNP + NAC 结合和海马中分离的 NAC 而增加。关于 CK 活性,AuNP 和 AuNP + NAC 关联在 CLP 后增加了两种大脑结构中的这一标志物。我们的数据提供了第一个实验证明,AuNP 和 NAC 联合能够通过减少神经炎症、氧化应激参数、线粒体功能障碍和 CK 活性来减少大鼠败血症诱导的脑功能障碍。复合物 I 活性随着 AuNP + NAC 结合和海马中分离的 NAC 而增加。关于 CK 活性,AuNP 和 AuNP + NAC 关联在 CLP 后增加了两种大脑结构中的这一标志物。我们的数据提供了第一个实验证明,AuNP 和 NAC 联合能够通过减少神经炎症、氧化应激参数、线粒体功能障碍和 CK 活性来减少大鼠败血症诱导的脑功能障碍。
更新日期:2020-04-20
down
wechat
bug