The objective of this study was to investigate variation in sperm quality metrics (motility, velocity, and concentration) in the critically endangered Mississippi gopher frog (Lithobates sevosus) over three sampling time points after a luteinizing hormone-releasing hormone analog (LHRHa) induction injection. Sperm was repeatedly collected from 11 individuals over three sampling times (30, 60, and 120 min) after injection. Variation in sperm quality was investigated using a repeated-measures mixed model approach. Repeated measures analyses of variance showed a significant effect of sampling time for percent motility and velocity. Concentration was found to be marginally related to sampling time, while progressive motility was not significantly related to time after injection. Our findings are important for optimizing assisted reproduction-related fertilization success and increasing the successful propagation of endangered species of imperiled frogs in captive breeding programs.

中文翻译：

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注射促黄体激素释放激素类似物的时间影响极度濒危的密西西比地鼠蛙 (Lithobates sevosus) 的精子质量

本研究的目的是调查极度濒危的密西西比地鼠蛙 (Lithobates sevosus) 在黄体生成素释放激素类似物 (LHRHa) 诱导注射后三个采样时间点精子质量指标（运动性、速度和浓度）的变化. 在注射后的三个采样时间（30、60 和 120 分钟）内从 11 个人重复收集精子。使用重复测量混合模型方法研究精子质量的变化。重复测量方差分析显示采样时间对运动百分比和速度有显着影响。发现浓度与采样时间略有相关，而渐进运动与注射后的时间没有显着相关。