Rosmarinic acid (RA), as a hydroxycinnamic acid ester of caffeic acid (CA) and 3,4-dihydroxyphenyllactic acid (3,4-DHPL), is a phenylpropanoid-derived plant natural product and has diverse biological activities. This work acts as a modular platform for microbial production using a two-cofactor (ATP and CoA) regeneration system to product RA based on a cell-free biosynthetic approach. Optimal activity of the reaction system was pH 8 and 30 °C. Total turnover number for ATP and CoA was 820.60 ± 28.60 and 444.50 ± 9.65, respectively. Based on the first hour data, the RA productivity reached 320.04 mg L-1 h-1 (0.889 mM L-1 h-1).

中文翻译：

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ATP和CoA双再生系统生产迷迭香酸


迷迭香酸（RA）是咖啡酸（CA）和3,4-二羟基苯基乳酸（3,4-DHPL）的羟基肉桂酸酯，是一种苯丙素衍生的植物天然产物，具有多种生物活性。这项工作作为微生物生产的模块化平台，使用双辅因子（ATP 和 CoA）再生系统基于无细胞生物合成方法生产 RA。反应体系的最佳活性为pH 8和30℃。 ATP 和 CoA 的总周转数分别为 820.60 ± 28.60 和 444.50 ± 9.65。根据第一小时的数据，RA 生产率达到 320.04 mg L-1 h-1 (0.889 mML-1 h-1)。