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Production of Rosmarinic Acid with ATP and CoA Double Regenerating System
Enzyme and Microbial Technology ( IF 3.4 ) Pub Date : 2019-12-01 , DOI: 10.1016/j.enzmictec.2019.109392
Yi Yan 1 , Pu Jia 2 , Yajun Bai 2 , Tai-Ping Fan 3 , Xiaohui Zheng 2 , Yujie Cai 1
Affiliation  

Rosmarinic acid (RA), as a hydroxycinnamic acid ester of caffeic acid (CA) and 3,4-dihydroxyphenyllactic acid (3,4-DHPL), is a phenylpropanoid-derived plant natural product and has diverse biological activities. This work acts as a modular platform for microbial production using a two-cofactor (ATP and CoA) regeneration system to product RA based on a cell-free biosynthetic approach. Optimal activity of the reaction system was pH 8 and 30 °C. Total turnover number for ATP and CoA was 820.60 ± 28.60 and 444.50 ± 9.65, respectively. Based on the first hour data, the RA productivity reached 320.04 mg L-1 h-1 (0.889 mM L-1 h-1).

中文翻译:


ATP和CoA双再生系统生产迷迭香酸



迷迭香酸(RA)是咖啡酸(CA)和3,4-二羟基苯基乳酸(3,4-DHPL)的羟基肉桂酸酯,是一种苯丙素衍生的植物天然产物,具有多种生物活性。这项工作作为微生物生产的模块化平台,使用双辅因子(ATP 和 CoA)再生系统基于无细胞生物合成方法生产 RA。反应体系的最佳活性为pH 8和30℃。 ATP 和 CoA 的总周转数分别为 820.60 ± 28.60 和 444.50 ± 9.65。根据第一小时的数据,RA 生产率达到 320.04 mg L-1 h-1 (0.889 mML-1 h-1)。
更新日期:2019-12-01
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