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Eco-efficient Biphasic Enzymatic Hydrolysis for the Green Production of Rare Baohuoside I
Enzyme and Microbial Technology ( IF 3.4 ) Pub Date : 2019-12-01 , DOI: 10.1016/j.enzmictec.2019.109431
Yuping Shen 1 , Man Wang 1 , Jinwei Zhou 1 , Yufei Chen 1 , Lili Xu 1 , Mengru Wu 1 , Guohua Xia 1 , James P Tam 2 , Jiangnan Yu 1 , Xiying Teng 1 , Huan Yang 1 , Xiaobin Jia 3
Affiliation  

Baohuoside I, a rare secondary glycoside from Epimedii Folium, has anti-osteoporosis and other pharmacological effects. In addition, it exhibits improved bioactivities compared with its original glycoside icariin. Conventional methods for the production of baohuoside I, such as acid hydrolysis, exhibit very low efficiency with serious pollution and substantial byproducts. The aim of this study was to develop an eco-efficient biphasic enzymatic hydrolysis system for the green production of rare baohuoside I. β-glucanase was selected to hydrolyze icariin and showed better performance than β-glucosidase. The biphasic system was constructed using HAc-NaAc buffer (pH 4.5) containing β-glucanase/icariin (Cicariin = 100 mg/mL; 1:1, w/w) and propyl acetate (1:2, v/v), and the hydrolysis was performed at 60℃ for 6 h. The hydrolysis capacity of icariin in the system was largely increased from 0.5 mg/mL to 100 mg/mL. The conversion ratio of icariin was greater than 99% in a 100-fold scale-up pilot test, demonstrating its strong potential for industrial applications. Furthermore, a process flow and production plant were designed to produce 1 ton of baohuoside I annually, and the profit would be approximately 4.5-fold its total cost. Biphasic enzymatic hydrolysis is an eco-efficient technology to produce baohuoside I and represents a promising method in the pharmaceutical industry.

中文翻译:

生态高效双相酶水解稀有宝火苷Ⅰ的绿色生产

宝火苷Ⅰ是一种罕见的淫羊藿次要苷,具有抗骨质疏松等药理作用。此外,与其原始的糖苷淫羊藿苷相比,它表现出更好的生物活性。传统的生产保活苷Ⅰ的方法,如酸水解,效率很低,污染严重,副产物多。本研究的目的是开发一种生态高效的双相酶水解系统,用于稀有宝火苷 I 的绿色生产。选择 β-葡聚糖酶来水解淫羊藿苷,其性能优于 β-葡糖苷酶。使用含有 β-葡聚糖酶/淫羊藿苷(Cicariin = 100 mg/mL;1:1,w/w)和乙酸丙酯(1:2,v/v)的 HAc-NaAc 缓冲液(pH 4.5)构建双相系统,并且水解在60℃进行6小时。系统中淫羊藿苷的水解能力从0.5 mg/mL大幅提高到100 mg/mL。淫羊藿苷在 100 倍放大中试试验中的转化率大于 99%,显示出其强大的工业应用潜力。此外,设计了一个工艺流程和生产装置,年产1吨保活苷Ⅰ,利润约为总成本的4.5倍。双相酶水解是一种生产保活苷 I 的生态高效技术,是制药行业中一种很有前景的方法。工艺流程及生产装置设计为年产1吨保活苷Ⅰ,利润约为总成本的4.5倍。双相酶水解是一种生产保活苷 I 的生态高效技术,是制药行业中一种很有前景的方法。工艺流程及生产装置设计为年产1吨保活苷Ⅰ,利润约为总成本的4.5倍。双相酶水解是一种生产保活苷 I 的生态高效技术,是制药行业中一种很有前景的方法。
更新日期:2019-12-01
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