L-tyrosine is an amino acid that has been widely used in the food, agriculture and pharmaceutical industries. In order to screen a tyrosine phenol-lyase (TPL) with excellent catalytic performance for L-tyrosine production, TPL genes from Citrobacter freundii (CfTPL), Erwinia herbicola (EhTPL) and Rhodobacter capsulatus (TutA) were codon-optimized and overexpressed in Escherichia coli. The results showed that EhTPL had the highest whole cell catalysis activity and tyrosine yield (3-fold that of CfTPL). The results of RT-qPCR and a stability analysis also revealed that EhTPL had a higher transcriptional level in whole cell catalysis, while CfTPL possessed greater stability. Conditions for the production by whole cell transformation were optimized in terms of reaction conditions and fed-batch strategy. Finally, the maximum production was obtained with a titer of 48.5 g·L-1 by intermittent feeding with a conversion ratio of 75%.

中文翻译：

---

通过全细胞生物转化方法使用酪氨酸苯酚裂解酶生产 L-酪氨酸

L-酪氨酸是一种广泛用于食品、农业和制药行业的氨基酸。为了筛选对 L-酪氨酸生产具有优异催化性能的酪氨酸苯酚裂解酶 (TPL)，对弗氏柠檬酸杆菌 (CfTPL)、草本欧文菌 (EhTPL) 和荚膜红杆菌 (TutA) 的 TPL 基因进行密码子优化并在大肠杆菌中过表达大肠杆菌。结果表明，EhTPL 具有最高的全细胞催化活性和酪氨酸产量（CfTPL 的 3 倍）。RT-qPCR 和稳定性分析的结果还表明，EhTPL 在全细胞催化中具有更高的转录水平，而 CfTPL 具有更高的稳定性。在反应条件和分批补料策略方面优化了全细胞转化的生产条件。最后，