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Preparation of cytochrome P450 enzyme-cobalt phosphate hybrid nano-flowers for oxidative coupling of benzylamine
Enzyme and Microbial Technology ( IF 3.4 ) Pub Date : 2019-12-01 , DOI: 10.1016/j.enzmictec.2019.109386
Kui Wu 1 , Ying Zhang 1 , Qingdi Sun 1 , Yuxin Chai 2 , Qian He 1 , Xiantai Zhou 2 , Xiaohui He 1 , Hongbing Ji 3
Affiliation  

A novel hybrid material with flower-mimicking morphology was fabricated with a facile coprecipitation method, and cytochrome P450 enzyme and cobalt phosphate were employed as organic and inorganic components, respectively. The hybrid nano-flowers showed excellent catalytic performance in the oxidative coupling of benzylamine, including the high conversion (99.9%) and selectivity (97.9%) in mild reaction conditions, as well as the satisfactory stability in the recycling experiments. Compared to free enzyme, the as-obtained materials exhibited enhanced activity. Such results indicate that the hybrid materials are potentially good candidates in the industrial enzyme catalysis.

中文翻译:

用于氧化偶联苄胺的细胞色素P450酶-磷酸钴杂化纳米花的制备

以细胞色素P450酶和磷酸钴分别作为有机和无机成分,采用简便的共沉淀法制备了一种具有仿花形态的新型杂化材料。杂化纳米花在苄胺氧化偶联中表现出优异的催化性能,包括在温和反应条件下的高转化率(99.9%)和选择性(97.9%),以及在循环实验中令人满意的稳定性。与游离酶相比,所获得的材料表现出增强的活性。这些结果表明杂化材料是工业酶催化中潜在的良好候选者。
更新日期:2019-12-01
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