The paramagnetic properties of metal ions and stable radicals can affect NMR spectra, which can lead to changes in peak intensities, relaxation times and chemical shifts. The changes from paramagnetic effects provide intriguing opportunities for solid-state NMR studies of proteins. In this review, we summarized the trends and progress of paramagnetic solid-state NMR of proteins in the past decade, and showed that paramagnetic effects have great potential applications for sensitivity enhancement, structure determination and topological analysis for microcrystalline proteins, protein complexes, protein aggregates and membrane proteins.

中文翻译：

---

蛋白质的顺磁性固态NMR。

金属离子和稳定基团的顺磁性会影响NMR光谱，从而导致峰强度，弛豫时间和化学位移发生变化。顺磁效应的变化为蛋白质的固态NMR研究提供了有趣的机会。在这篇综述中，我们总结了蛋白质在过去十年中顺磁性固态NMR的趋势和进展，并表明顺磁性效应对于微晶蛋白质，蛋白质复合物，蛋白质聚集体的敏感性增强，结构确定和拓扑分析具有巨大的潜在应用。和膜蛋白。