Objective: To investigate the effects of propofol used in early pregnancy on brain development and function of offspring, and further to explore the effects of docosahexaenoic acid (DHA) intervention. Methods: Forty pregnant rats were randomly divided into four groups: control group (C), propofol group (P), DHA intervention group (D), and propofol + DHA group (P + D). The DHA treatment was before propofol was administered. Morris water maze test was performed 30 days after delivery. The levels of amyloid beta (Aβ), IL-1β and reactive oxygen species (ROS) in hippocampus were detected by enzyme-linked immunosorbent assay (ELISA). The expression of brain-derived neurotrophic factor (BDNF) and tyrosine kinase-B (Trk-B), protein kinase B (Akt), p-Akt and cAMP response element-binding protein (CREB) in hippocampus were detected by western blot. Results: The learning and memory abilities of the rats in P group were reduced. The levels of Aβ, IL-1β and ROS were increased, while the levels of BDNF, Trk-B and CREB, and p-Akt/Akt ratio were reduced. In addition, compared with P group, DHA in P + D group reversed or alleviated adverse changes caused by propofol. Conclusions: Application of general anesthesia with propofol during the early stage of pregnancy can negatively affect the brain development of the offspring to reduce the learning and memory ability, while DHA can reverse it.

中文翻译：

---

妊娠初期全麻联合丙泊酚麻醉对SD大鼠后代脑发育和功能的影响以及DHA的干预。

目的：探讨孕早期使用异丙酚对脑发育和后代功能的影响，并探讨二十二碳六烯酸（DHA）干预的作用。方法：40只妊娠大鼠随机分为四组：对照组（C），丙泊酚组（P），DHA干预组（D）和丙泊酚+ DHA组（P + D）。DHA治疗是在服用异丙酚之前进行的。分娩后30天进行莫里斯水迷宫测试。通过酶联免疫吸附试验（ELISA）检测海马中的淀粉样蛋白β（Aβ），IL-1β和活性氧（ROS）的水平。Western blot检测海马中脑源性神经营养因子（BDNF）和酪氨酸激酶-B（Trk-B），蛋白激酶B（Akt），p-Akt和cAMP反应元件结合蛋白（CREB）的表达。结果：P组大鼠学习记忆能力下降。Aβ，IL-1β和ROS水平升高，而BDNF，Trk-B和CREB水平以及p-Akt / Akt比降低。另外，与P组相比，P + D组的DHA逆转或减轻了异丙酚引起的不良变化。结论：孕早期使用丙泊酚全麻可对后代的大脑发育产生负面影响，从而降低其学习和记忆能力，而DHA可逆转后者。