OBJECTIVES The active experimental autoimmune encephalomyelitis (EAE) model is often initiated using myelin oligodendrocyte glycoprotein (MOG) immunization followed by pertussis toxin (PTX) to study multiple sclerosis. However, PTX inactivates G protein-coupled receptors, and with increasing knowledge of the role that various G protein-coupled receptors play in immune homeostasis, it is valuable to establish neuroimmune endpoints for active EAE without PTX. METHODS Female C57BL/6 mice were immunized with MOG35-55 peptide in Complete Freund's Adjuvant and neuroinflammation, including central nervous system B-cell infiltration, was compared to saline-injected mice. Since it was anticipated that disease onset would be slower and less robust than EAE in the presence of PTX, both cervical and lumbosacral sections of the spinal cord were evaluated. RESULTS Immunohistochemical analysis showed that EAE without PTX induced immune infiltration, CCL2 and VCAM-1 upregulation. Demyelination in the cervical region correlated with the infiltration of CD19+ B cells in the cervical region. There was upregulation of IgG, CD38, and PDL1 on B cells in cervical and lumbosacral regions of the spinal cord in EAE without PTX. Interestingly, IgG was expressed predominantly by CD19- cells. CONCLUSIONS These data demonstrate that many neuroimmune endpoints are induced in EAE without PTX and although clinical disease is mild, this can be used as an autoimmune model when PTX inactivation of G protein-coupled receptors is not desired.

中文翻译：

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在缺乏百日咳毒素的实验性自身免疫性脑脊髓炎中，脊髓颈椎和腰ac神经区的神经炎症和B细胞表型。

目的主动实验性自身免疫性脑脊髓炎（EAE）模型通常使用髓磷脂少突胶质细胞糖蛋白（MOG）免疫接种，然后百日咳毒素（PTX）免疫来研究多发性硬化症。然而，PTX使G蛋白偶联受体失活，并且随着对各种G蛋白偶联受体在免疫稳态中所起作用的了解的增加，为没有PTX的活性EAE建立神经免疫终点非常有价值。方法用完全弗氏佐剂中的MOG35-55肽对雌性C57BL / 6小鼠进行免疫，并与注射盐水的小鼠比较神经炎症，包括中枢神经系统B细胞浸润​​。由于预计在PTX存在下疾病发作会比EAE慢且不那么强壮，因此对脊髓的颈椎和腰s部进行了评估。结果免疫组织化学分析显示，无PTX的EAE诱导免疫浸润，CCL2和VCAM-1上调。子宫颈区域的脱髓鞘与子宫颈区域的CD19 + B细胞的浸润相关。在没有PTX的EAE中，脊髓的颈和腰s部B细胞的IgG，CD38和PDL1上调。有趣的是，IgG主要由CD19-细胞表达。结论这些数据表明，在没有PTX的EAE中诱导了许多神经免疫终点，尽管临床疾病较轻，当不需要PTX失活G蛋白偶联受体时，可以将其用作自身免疫模型。子宫颈区域的脱髓鞘与子宫颈区域的CD19 + B细胞的浸润相关。在没有PTX的EAE中，脊髓的颈和腰s部B细胞的IgG，CD38和PDL1上调。有趣的是，IgG主要由CD19-细胞表达。结论这些数据表明，在没有PTX的EAE中诱导了许多神经免疫终点，尽管临床疾病较轻，当不需要PTX失活G蛋白偶联受体时，可以将其用作自身免疫模型。子宫颈区域的脱髓鞘与子宫颈区域的CD19 + B细胞的浸润相关。在没有PTX的EAE中，脊髓的颈和腰s部B细胞的IgG，CD38和PDL1上调。有趣的是，IgG主要由CD19-细胞表达。结论这些数据表明，在没有PTX的EAE中诱导了许多神经免疫终点，尽管临床疾病较轻，但当不需要PTX失活G蛋白偶联受体时，可以将其用作自身免疫模型。