Objectives: The aim of the study was to determine the relationships between microRNA-20a and microRNA-125b expression and apoptosis and inflammation in a rat model of spinal cord injury (SCI) using microscopy, immunohistochemistry, and molecular biology. Methods: Sixty-one rats were divided into three groups: a control group that was not subjected to any operation; a sham-operated group; and an experimental group that was subjected to spinal cord compression. The experimental group was further subdivided into two subgroups: the experimental control group, which did not receive any drug treatment; and the methylprednisolone treatment group, which received 30 mg/kg methylprednisolone on day 0 followed by 10 mg/kg/day methylprednisolone from days 1-14. Results: Tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 levels increased in the experimental control group on days 1 and 3, and decreased in the experimental control group and methylprednisolone treatment group on days 7 and 14. Caspase-3 levels increased in the experimental control group on day 1, and decreased in the experimental control group and methylprednisolone treatment group on days 3, 7, and 14. MicroRNA-20a expression was upregulated in the experimental control group on days 1 and 3, and microRNA-125b expression was downregulated on days 3 and 7. Conclusions: After SCI, upregulated microRNA-20a expression and increased proinflammatory cytokines may lead to an increase in inflammation. MicroRNA-125b may be associated with caspase-3, and microRNA-125b downregulation may inhibit apoptosis. Although the results of this study suggest potential relationships between microRNA-20a and microRNA-125b expression and apoptosis and inflammation in SCI, further studies are needed to confirm microRNA-20a and microRNA-125b as biomarkers in SCI and to develop new strategies for the treatment of SCI.

中文翻译：

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实验性脊髓损伤中microRNA-20a和microRNA-125b表达与细胞凋亡和炎症的关系。

目的：本研究的目的是利用显微镜，免疫组织化学和分子生物学方法确定大鼠脊髓损伤（SCI）模型中microRNA-20a和microRNA-125b表达与凋亡和炎症之间的关系。方法：61只大鼠分为三组：不进行任何手术的对照组；不进行任何手术的对照组。伪造的团体；实验组接受脊髓压迫。实验组又分为两个亚组：实验对照组，不接受任何药物治疗；对照组，不接受任何药物治疗。甲基强的松龙治疗组，在第0天接受30 mg / kg的甲基强的松龙，然后从1-14天开始接受10 mg / kg /天的甲基强的松龙。结果：在第1天和第3天，实验对照组的肿瘤坏死因子-α（TNF-α）和白介素（IL）-6水平升高，而在第7天和第14天，实验对照组和甲基泼尼松龙治疗组则降低。在第1天，实验对照组的血脂水平升高，在第3、7和14天，实验对照组和甲基泼尼松龙治疗组的血脂水平降低。在第1天和第3天，实验对照组的MicroRNA-20a表达上调，而microRNA -125b表达在第3天和第7天下调。结论：SCI后，microRNA-20a表达上调和促炎细胞因子增加可能导致炎症增加。MicroRNA-125b可能与caspase-3相关，而microRNA-125b的下调可能会抑制细胞凋亡。