ABSTRACT 2H/1H ratios in animal biomass reflect isotopic input from food and water. A 10-week controlled laboratory study raised 48 mice divided in two generations (8 mothers Mus musculus and their offspring). The mice were divided into four groups based on the combination of 2H, 13C, 15N-enriched and non-enriched food and water. Glycine, the most common amino acid in bone collagen, carried the 2H, 13C, 15N-isotopic spike in food. ANOVA data analysis indicated that hydrogen in food accounted for ∼81 % of the hydrogen isotope inventory in collagen whereas drinking water hydrogen contributed ∼17 %. Air humidity contributed an unspecified amount. Additionally, we monitored 13C and 15N-enrichment in bone collagen and found strong linear correlations with the 2H-enrichment. The experiments with food and water indicate two biosynthetic pathways, namely (i) de novo creation of non-essential amino acids using hydrogen from water, and (ii) the integration of essential and non-essential amino acids from food. The lower rate of isotope uptake in mothers’ collagen relative to their offspring indicates incomplete bone collagen turnover after ten weeks. The variance of hydrogen stable isotope ratios within the same cohort may limit its usefulness as a single sample proxy for archaeological or palaeoenvironmental research.

中文翻译：

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小鼠骨胶原中 H、C 和 N 的稳定同位素作为同位素控制的食物和水摄入量的反映

摘要 动物生物量中的 2H/1H 比率反映了来自食物和水的同位素输入。一项为期 10 周的对照实验室研究将 48 只小鼠分成两代（8 只母鼠和它们的后代）。根据 2H、13C、15N 富集和非富集食物和水的组合，将小鼠分为四组。甘氨酸是骨胶原中最常见的氨基酸，在食物中携带 2H、13C、15N 同位素峰值。方差分析数据分析表明，食物中的氢占胶原蛋白氢同位素总量的 81%，而饮用水中的氢占 17%。空气湿度贡献了不确定的数量。此外，我们监测了骨胶原中的 13C 和 15N 富集，发现与 2H 富集有很强的线性相关性。食物和水的实验表明了两种生物合成途径，即（i）使用来自水中的氢从头制造非必需氨基酸，以及（ii）整合来自食物的必需氨基酸和非必需氨基酸。相对于其后代，母亲胶原蛋白中同位素吸收率较低表明十周后骨胶原蛋白的更新不完全。同一队列中氢稳定同位素比率的差异可能会限制其作为考古或古环境研究的单一样本代表的有用性。