This study investigated the synergistic effects of combined chlorine (200, 500, 700, and 1000 ppm) and vitamin B₁ (1000, 2000, and 3000 ppm) on the murine norovirus-1 (MNV-1), a human norovirus (NoV) surrogate, on oyster surface. Vitamin B₁ slightly reduced MNV-1 (0.04–0.3 log-reduction), whereas chlorine significantly reduced MNV-1 (0.4–1.0 log-reduction). The combined chlorine and vitamin B₁ resulted in a 0.52–1.97 log-reduction of MNV-1. The synergistic reduction in the MNV titer was not dependent on the concentrations of chlorine and vitamin B₁, and it ranged between 0.08 and 1.03 log₁₀ PFU/mL. The largest synergistic reduction observed was for the combined 700 ppm chlorine and 1000 ppm vitamin B₁. The pH and mechanical texture of the oysters were not significantly changed by the combined 0–1000 ppm chlorine and 3000 ppm vitamin B₁. The overall sensory acceptability were significantly (P < 0.05) reduced in oysters treated with 1000 ppm chlorine and 3000 ppm vitamin B₁ than in those treated with 0–700 ppm chlorine and 3000 ppm vitamin B₁. This study suggests that the combined 700 ppm chlorine and 3000 ppm vitamin B₁ could potentially be used to reduce NoV on oyster surface without causing concomitant changes in the mechanical texture, pH, or sensory qualities of the oysters.

中文翻译：

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氯和维生素B1的协同作用对减少牡蛎（Crassostrea gigas）表面鼠诺罗病毒1的协同作用。

这项研究调查了氯（200、500、700和1000 ppm）和维生素B ₁（1000、2000和3000 ppm）的组合对鼠诺如病毒1（MNV-1），人诺如病毒（NoV）的协同作用）在牡蛎表面上替代。维生素B ₁轻微降低MNV-1（减少0.04-0.3对数），而氯显着减少MNV-1（减少0.4-1.0对数）。氯和维生素B ₁的结合导致MNV-1的对数减少0.52-1.97。MNV滴度的协同降低并不取决于氯和维生素B ₁的浓度，其范围在0.08至1.03 log ₁₀ PFU / mL之间。观察到的最大协同增效作用是将700 ppm氯和1000 ppm维生素B合并使用₁。0-1000 ppm的氯和3000 ppm的维生素B ₁的组合，牡蛎的pH值和机械质地没有明显变化。与用0–700 ppm氯和3000 ppm维生素B ₁处理的 牡蛎相比，用1000 ppm氯和3000 ppm维生素B ₁处理的牡蛎的总体感觉可接受性显着降低（P <0.05）。这项研究表明，结合使用700 ppm的氯和3000 ppm的维生素B ₁可以潜在地降低牡蛎表面的NoV，而不会引起牡蛎的机械质地，pH或感官品质的变化。