Objectives: miR-200c-3p has been shown to serve as a tumor suppressor in various tumor types. However, the biological function of miR-200c-3p in nephroblastoma remains unknown. This study aims to investigate the biological function and regulatory mechanisms of miR-200c-3p in nephroblastoma development. Methods: The expression of miR-200c-3p in nephroblastoma tissues and cells was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of miR-200c-3p on the proliferation and cell cycle of SK-NEP-1 nephroblastoma cell line were evaluated by CCK-8 assay, colony formation assay, and flow cytometry. The effects of miR-200c-3p on the migratory and invasive capacities of SK-NEP-1 cells were measured by wound healing assay and transwell assay. The ability of miR-200c-3p to target fibroblast growth factor receptor substrate 2 (FRS2) was detected by quantitative PCR, western blot, and luciferase reporter assay. Results: The expression of miR-200c-3p was significantly downregulated in nephroblastoma tissues and cells compared with that in normal renal tissues and cells. miR-200c-3p inhibited the proliferative, migratory, and invasive capacities of nephroblastoma cells by targeting FRS2. Conclusions: miR-200c-3p suppresses the malignant behaviors of nephroblastoma cells by downregulating the expression of FRS2.

中文翻译：

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miR-200c-3p通过靶向FRS2抑制肾母细胞瘤细胞的增殖，迁移和侵袭能力。

目标：miR-200c-3p已被证明可在多种肿瘤类型中发挥抑癌作用。但是，miR-200c-3p在肾母细胞瘤中的生物学功能仍然未知。本研究旨在探讨miR-200c-3p在肾母细胞瘤发展中的生物学功能和调控机制。方法：采用定量实时聚合酶链反应（qRT-PCR）检测miR-200c-3p在肾母细胞瘤组织和细胞中的表达。通过CCK-8试验，集落形成试验和流式细胞术评估了miR-200c-3p对SK-NEP-1肾母细胞瘤细胞系增殖和细胞周期的影响。通过伤口愈合测定和transwell测定，测定miR-200c-3p对SK-NEP-1细胞迁移和侵袭能力的影响。miR-200c-3p靶向成纤维细胞生长因子受体底物2（FRS2）的能力通过定量PCR，蛋白质印迹和萤光素酶报告基因检测法进行检测。结果：与正常肾组织和细胞相比，肾母细胞瘤组织和细胞中miR-200c-3p的表达明显下调。miR-200c-3p通过靶向FRS2抑制肾母细胞瘤细胞的增殖，迁移和侵袭能力。结论：miR-200c-3p通过下调FRS2的表达抑制肾母细胞瘤细胞的恶性行为。靶向FRS2的肾母细胞瘤细胞的迁移和侵袭能力。结论：miR-200c-3p通过下调FRS2的表达抑制肾母细胞瘤细胞的恶性行为。靶向FRS2的肾母细胞瘤细胞的迁移和侵袭能力。结论：miR-200c-3p通过下调FRS2的表达抑制肾母细胞瘤细胞的恶性行为。