We investigated the effects of ibandronate, a bisphosphonate; eldecalcitol, an active vitamin D3 analogue; and combination treatment with both agents on secondary osteoporosis and arthritis using rats with adjuvant-induced arthritis. Arthritis was induced in 8-week-old male Lewis rats. Rats were randomized into four treatment groups and an untreated normal control group: ibandronate, eldecalcitol, ibandronate + eldecalcitol, vehicle, and control. Paw thickness was measured to evaluate arthritis. Joint destruction was evaluated histomorphometrically by the ankle joint stained with Fast Green and safranin O. The femur and lumbar spine were scanned using dual-energy X-ray absorptiometry, and the distal femur was scanned using micro-computed tomography for bone mineral density (BMD) and trabecular microstructural evaluations. Ibandronate and/or eldecalcitol increased BMD in both the lumbar vertebrae and femur and improved several microstructural parameters (bone volume/total volume, structure model index, trabecular number, and trabecular separation of the distal femur). In addition, there was an additive effect of combination treatment compared with single treatments for most trabecular parameters, including BMD and bone volume. However, ibandronate and/or eldecalcitol did not inhibit arthritis and joint destruction. Combination treatment with ibandronate and eldecalcitol may be effective for secondary osteoporosis associated with arthritis.

中文翻译：

---

Micro-CT成像分析伊班膦酸和去骨钙化醇对佐剂性关节炎大鼠继发性骨质疏松和关节炎的影响。

我们研究了双膦酸盐伊班膦酸盐的作用。降钙素醇，一种活性维生素D3类似物；并使用佐剂诱发的关节炎的大鼠对继发性骨质疏松症和关节炎的两种药物联合治疗。在8周大的雄性Lewis大鼠中诱发了关节炎。将大鼠随机分为四个治疗组和一个未治疗的正常对照组：伊班膦酸，去骨钙糖醇，伊班膦酸+去骨钙糖醇，赋形剂和对照组。测量足的厚度以评估关节炎。用Fast Green和番红花O染色的踝关节进行组织破坏形态学评估。用双能X线骨密度仪扫描股骨和腰椎，用微计算机断层扫描术扫描股骨远端的骨密度（BMD） ）和小梁的微结构评估。伊班膦酸和/或降钙糖醇可增加腰椎和股骨的骨密度，并改善了一些微结构参数（骨体积/总体积，结构模型指数，小梁数和股骨远端的小梁分离）。此外，对于大多数小梁参数（包括BMD和骨量），与单次治疗相比，联合治疗具有加和作用。然而，伊班膦酸和/或去骨钙化醇不能抑制关节炎和关节破坏。伊班膦酸和去骨钙化醇的联合治疗可能有效治疗与关节炎有关的继发性骨质疏松症。此外，对于大多数小梁参数（包括BMD和骨量），与单次治疗相比，联合治疗具有加和作用。但是，伊班膦酸和/或去骨钙化醇不能抑制关节炎和关节破坏。伊班膦酸和去骨钙化醇的联合治疗可能有效治疗与关节炎有关的继发性骨质疏松症。此外，对于大多数小梁参数（包括BMD和骨量），与单次治疗相比，联合治疗具有加和作用。然而，伊班膦酸和/或去骨钙化醇不能抑制关节炎和关节破坏。伊班膦酸和去骨钙化醇的联合治疗可能有效治疗与关节炎有关的继发性骨质疏松症。