We recently reported the application of human menstrual blood stem cells' (HuMenSCs) transplantation as a treatment modality in a rat model of premature ovarian failure (POF). We continued to investigate further in this respect. Female rats were injected intraperitoneally with 36 mg/kg busulfan. HuMenSCs were obtained, grown, and analyzed for immunophenotypic features at passage three. The cells were labeled with CM-Dil and infused into the rats. There were four groups: normal, negative control, treatment, and Sham. One month after treatment, the ovaries were collected and weighed. Histological sections were prepared from the ovary and HuMenSCs were tracking. Subsequently, we examined the changes of expression of Bax and B cell lymphoma 2 (Bcl2) genes by real-time polymerase chain reaction assay. One month after HuMenSCs transplantation, these cells were located in the ovarian interstitium and granulosa cells (GCs). The number of TUNEL-positive cells significantly decreased in the treatment group. Also the expression level of Bax genes, unlike Bcl2 gene, significantly decreased compared with negative and sham groups. In our study, HuMenSCs were tracked in ovarian tissues within 2 months after transplantation, and they differentiated into GCs. Therefore, the use of these cells can be a practical and low-cost method for the treatment of POF patients.

中文翻译：

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人类月经血干细胞来源的颗粒细胞参与卵巢早衰的体内大鼠模型中的卵泡形成。

我们最近报道了人类经血干细胞（HuMenSCs）移植在卵巢早衰（POF）大鼠模型中作为治疗方式的应用。我们在这方面继续进行进一步调查。给雌性大鼠腹膜内注射36mg / kg的白消安。在第3代中获得HuMenSCs，使其生长并进行免疫表型分析。用CM-Dil标记细胞并注入大鼠中。有四组：正常，阴性对照，治疗和假手术。治疗后一个月，收集卵巢并称重。从卵巢中制备组织切片，并对HuMenSCs进行跟踪。随后，我们通过实时聚合酶链反应分析检查了Bax和B细胞淋巴瘤2（Bcl2）基因表达的变化。HuMenSCs移植一个月后，这些细胞位于卵巢间质和颗粒细胞（GC）中。在治疗组中，TUNEL阳性细胞的数量显着减少。与阴性和假组相比，与Bcl2基因不同，Bax基因的表达水平也明显降低。在我们的研究中，HuMenSCs在移植后2个月内在卵巢组织中被追踪，并分化为GC。因此，这些细胞的使用可能是治疗POF患者的一种实用且低成本的方法。HuMenSCs在移植后2个月内在卵巢组织中被追踪，并分化为GC。因此，这些细胞的使用可能是治疗POF患者的一种实用且低成本的方法。HuMenSCs在移植后2个月内在卵巢组织中被追踪，并分化为GC。因此，这些细胞的使用可能是治疗POF患者的一种实用且低成本的方法。