Myrciaria dubia is a native plant from the Amazon region which produces red-purplish fruit rich in antioxidant compounds such as ascorbic acid, carotenoids, and phenolic. M. dubia fruit is used to prepare juices considered to possess high nutritional content providing health benefits. The aim of this study was to examine the ability of M. dubia juice to protect DNA against genomic instability induced by sub-acute ethanol consumption attributed to oxidative stress. Mice were treated for 28 days with juice at 25% and 50% diluted in distilled water or with the diluted combination juice plus ethanol (5 g/kg). The genotoxic/antigenotoxic and mutagenic/antimutagenic effects were assessed using comet assay in blood, liver, and kidney and micronucleus (MN) test with bone marrow. In addition, the mutagenicity was also evaluated using Salmonella/microsome assay. Phytochemical compounds were determined using HPLC/PDA/MS/MS. The juice did not induce genotoxic effects in blood, kidney, and liver cells at both doses. In combination with ethanol, the juice reduced the alcohol-mediated DNA damage in all tissues analyzed. Further, the juice did not produce mutagenic effects and decreased mutagenicity induced by ethanol in the bone marrow. The anthocyanins were major compounds detected by HPLC/PDA/MS/MS, which modulated genotoxic and mutagenic effects initiated by ethanol and at least in part appeared responsible for the observed antigenotoxic and antimutagenic effects of M. dubia juice.

中文翻译：

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杜仲肉汁对乙醇28天治疗的小鼠的抗遗传和抗诱变作用。

杜拜Myrciaria dubia是一种来自亚马逊地区的本地植物，其产生的红紫色水果富含抗氧化剂，如抗坏血酸，类胡萝卜素和酚类。杜比亚分枝杆菌果实用于制备被认为具有高营养含量并提供健康益处的果汁。这项研究的目的是研究杜鹃分枝杆菌汁保护DNA免受因氧化应激引起的亚急性乙醇消耗而引起的基因组不稳定的能力。用在蒸馏水中稀释了25％和50％的果汁或用稀释的果汁加乙醇（5 g / kg）的混合液处理小鼠28天。在彗星，血液，肝脏，肾脏和微核（MN）试验中，使用彗星试验评估了遗传毒性/抗遗传毒性和诱变/抗诱变作用。此外，还使用沙门氏菌/微粒体测定法评估了致突变性。使用HPLC / PDA / MS / MS测定植物化学化合物。两种剂量的果汁都不会在血液，肾脏和肝细胞中引起遗传毒性作用。与乙醇结合使用时，果汁减少了所分析的所有组织中酒精介导的DNA损伤。此外，该汁液不产生诱变作用并且降低了乙醇在骨髓中诱导的诱变性。花色苷是通过HPLC / PDA / MS / MS检测到的主要化合物，其调节由乙醇引发的遗传毒性和诱变作用，并且至少部分地表现出所观察到的杜比亚分支杆菌汁液的抗原毒性和抗诱变作用。与乙醇结合使用时，果汁减少了所分析的所有组织中酒精介导的DNA损伤。此外，该汁液不产生诱变作用并且降低了乙醇在骨髓中诱导的诱变性。花色苷是通过HPLC / PDA / MS / MS检测到的主要化合物，其调节由乙醇引发的遗传毒性和诱变作用，并且至少部分地表现出所观察到的杜比亚分支杆菌汁液的抗原毒性和抗诱变作用。与乙醇结合使用时，果汁减少了所分析的所有组织中酒精介导的DNA损伤。此外，该汁液不产生诱变作用并且降低了乙醇在骨髓中诱导的诱变性。花色苷是通过HPLC / PDA / MS / MS检测到的主要化合物，其调节由乙醇引发的遗传毒性和诱变作用，并且至少部分地表现出所观察到的杜比亚分支杆菌汁液的抗原毒性和抗诱变作用。