MicroRNAs (miRNAs) are involved in various crucial biological processes including regulation of cell differentiation, proliferation, and migration, and are closely associated with tumor development. This study aimed to investigate miR-130b expression levels in lung cancer patient tissues. Two Gene Expression Omnibus (GEO) databases, including GSE48414 and GSE74190, and two The Cancer Genome Atlas (TCGA) databases including TCGA LUAD and TCGA LUSC, were accessed to obtain information for differential expression analysis and clinical-pathological correlation analysis. The results showed that miR-130b expression levels were significantly increased in lung cancer compared to normal tissues. Data also demonstrated that confounding factors such as tumor clinical stages and tumor invasion depth markedly affected miR-130b expression levels in cancer patients. A total of 169 target genes modified by miR-130b expression were identified by using 4 online websites for target gene prediction. Further enrichment analysis indicated that these 169 target genes were significantly enriched in several cancer-related biological processes and signaling pathways, including wound healing, cell proliferation, Wnt signaling, Ras signaling, and mTOR signaling. It was also of interest to examine the seven sites on the promoter region of miR-130b encoding gene in lung cancer patients and then compare methylation at these loci with miR-130b expression. The correlation analysis between encoding gene methylation and miR-130b expression in TCGA datasets revealed that decreased methylation in the promoter region was significantly associated with elevated miR-130b expression. This phenomenon was markedly dependent upon smoking history and clinical-pathological features. In conclusion, data indicated alterations in the methylation of DNA promoter region of miR-130b encoding gene were associated with disturbances in miR-130b expression in lung cancer patients suggesting that the DNA methylation process and miR-130b expression may serve as biomarkers for detection of lung cancer.

中文翻译：

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使用生物信息学分析，DNA甲基化调节miR-130b在人类肺癌中的表达。

MicroRNA（miRNA）参与各种关键的生物学过程，包括调节细胞分化，增殖和迁移，并且与肿瘤的发展密切相关。这项研究旨在调查肺癌患者组织中miR-130b的表达水平。访问了两个基因表达综合数据库（GEO），包括GSE48414和GSE74190，以及两个癌症基因组图谱（TCGA）数据库，包括TCGA LUAD和TCGA LUSC，以获得差异表达分析和临床病理相关性分析的信息。结果表明，与正常组织相比，肺癌中的miR-130b表达水平显着增加。数据还表明，诸如肿瘤临床分期和肿瘤浸润深度之类的混杂因素显着影响了癌症患者中miR-130b的表达水平。通过使用4个在线网站进行目标基因预测，总共鉴定出169个被miR-130b表达修饰的目标基因。进一步的富集分析表明，这169个靶基因在几种与癌症相关的生物学过程和信号传导途径中显着富集，包括伤口愈合，细胞增殖，Wnt信号传导，Ras信号传导和mTOR信号传导。检查肺癌患者中miR-130b编码基因的启动子区域上的七个位点，然后将这些基因座处的甲基化与miR-130b的表达进行比较，也很有意义。TCGA数据集中的编码基因甲基化与miR-130b表达之间的相关分析表明，启动子区域甲基化的降低与miR-130b表达的升高显着相关。这种现象明显取决于吸烟史和临床病理特征。总之，数据表明miR-130b编码基因的DNA启动子区域甲基化的改变与肺癌患者miR-130b表达的紊乱有关，表明DNA甲基化过程和miR-130b表达可能是检测肝癌的生物标志物。肺癌。