Grapholita molesta is one of the most destructive fruit pests distributed worldwide. Odorant receptors (ORs) located on the dendritic membrane of chemosensory neurons are deemed to be key molecules for sensing exogenous chemical signals. In this study, GmolOR9, a general OR from G. molesta, was functionally characterized. Quantitative real‐time polymerase chain reaction revealed that GmolOR9 was more highly expressed in adults than in other stages, including eggs, larvae, and pupae. GmolOR9 expression was highly significantly more in the antennae of females than in those of males, and the highest level occurred in the antennae of 3‐day‐old female adults. GmolOR9 was broadly tuned to eight of 47 odorant components tested, including (Z)‐3‐hexenyl acetate, butyl propionate, ethyl hexanoate, ethyl heptanoate, 1‐hexanol, (Z)‐3‐hexenol, 2‐ethyl‐1‐hexanol, and linalool, by in vitro heterologous expression. Furthermore, electroantennogram responses indicated that the effects of dsOR9‐injected females to (Z)‐3‐hexenyl acetate dramatically decreased. These results suggested that GmolOR9 might be involved in detecting host‐plant volatiles. Moreover, (Z)‐3‐hexenyl acetate might serve as a potential attractant for the biological control of G. molesta.

中文翻译：

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来自Grapholitamoleta（鳞翅目：Tortricidae）的一般气味受体GmolOR9主要调节到八种寄主植物挥发物

蝾螈是世界范围内最具破坏性的水果害虫之一。位于化学感应神经元树突膜上的气味受体（ORs）被认为是感知外源化学信号的关键分子。在这项研究中，GmolOR9，一种来自 G.moleta 的通用 OR，在功能上被表征。定量实时聚合酶链反应显示 GmolOR9 在成虫中的表达高于其他阶段，包括卵、幼虫和蛹。GmolOR9在雌性触角中的表达明显高于雄性，并且在3日龄雌性成虫的触角中表达水平最高。GmolOR9 被广泛调整为测试的 47 种气味成分中的 8 种，包括 (Z)-3-己烯基乙酸酯、丙酸丁酯、己酸乙酯、庚酸乙酯、1-己醇、(Z)-3-己烯醇、2-乙基-1-己醇和芳樟醇，通过体外异源表达。此外，触角电图反应表明注射 dsOR9 的雌性对 (Z)-3-己烯基乙酸酯的影响显着降低。这些结果表明 GmolOR9 可能参与检测宿主植物挥发物。此外，(Z)-3-己烯基乙酸酯可作为潜在的引诱剂用于 G.moleta 的生物控制。