The rotating wall vessel (RWV) bioreactor is a powerful tool for the generation of sizeable, faster-growing organoids. However, the ideal, low-shear, modeled microgravity environment in the RWV is frequently disrupted by the formation of bubbles, a critical but understated failure mode. To address this, we have designed and fabricated a novel, modified RWV bioreactor capable of continuously removing bubbles while providing optimal fluid dynamics. We validated the capacity of this device with computational and empirical studies. We anticipate that our novel bioreactor will be more consistent and easier to use and may fill a unique and unmet niche in the burgeoning field of organoids.

中文翻译：

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用于改善球体/类器官形成的气泡隔离旋转壁容器生物反应器。


旋转壁容器 (RWV) 生物反应器是生成大型、生长更快的类器官的强大工具。然而，RWV 中理想的低剪切模拟微重力环境经常因气泡的形成而受到破坏，这是一种关键但低调的失效模式。为了解决这个问题，我们设计并制造了一种新型、改良的 RWV 生物反应器，能够连续去除气泡，同时提供最佳的流体动力学。我们通过计算和实证研究验证了该设备的能力。我们预计我们的新型生物反应器将更加一致且更易于使用，并且可能会填补新兴类器官领域中独特且未满足的利基市场。