One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid metabolism and exceptional regulatory importance in the control of almost all aspects of cellular functions created the need for tools to visualize their localizations and dynamics at the single cell level. However, there is also an increasing need for methods to determine the cellular distribution of other lipids regulatory or structural, such as diacylglycerol, phosphatidic acid, or other phospholipids and cholesterol. This review will summarize recent advances in this research field focusing on the means by which changes can be described in more quantitative terms.

中文翻译：

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使用脂质结合蛋白结构域定量各种膜区室中的脂质变化。

细胞生物学的最大挑战之一是绘制各种细胞器膜的脂质组成图，并确定控制脂质在细胞区室之间合成和分布的过程的确切位置。磷酸肌醇，具有快速代谢功能的低丰度脂质以及在控制细胞功能的几乎所有方面方面都具有异常重要的调节作用的关键作用，产生了对在单个细胞水平上可视化其定位和动态的工具的需求。但是，也越来越需要确定其他调节或结构性脂质的细胞分布的方法，例如二酰基甘油，磷脂酸或其他磷脂和胆固醇。