OBJECTIVE Weight gain after kidney transplantation (Tx) is considered a risk factor for poor outcomes. Increased oxidative stress is associated with not only chronic renal disease and Tx, but also obesity and cardiovascular disease. The aim of this pilot study was to test whether oxidative stress is related to weight gain at 12-months after kidney Tx and to obtain preliminary insight into potential mechanisms involved. DESIGN & METHODS Recipients (n=33) were classified into two groups; weight loss and weight gain, based on their weight changes at 12-months post-transplant. Total antioxidant capacity (TAOC) and lipid peroxidation (TBARS) were measured to evaluate oxidative stress from plasma at baseline and 12-months. A secondary data analysis was conducted to identify potential gene regulation. RESULTS Seventeen recipients lost (-6.63±5.52kg), and sixteen recipients gained weight (8.94±6.18kg). TAOC was significantly decreased at 12-months compared to baseline for the total group, however, there was no significant difference between groups at either time point. TBARS was higher in weight gain group, at both time points, and it was significantly higher at 12-months (p=0.012). Gene expression profiling analysis showed that 7 transcripts annotated to reactive oxygen species related genes in adipose tissue were expressed significantly lower in weight gain group at baseline, which might be a negative feedback mechanism to reduce oxidative stress. CONCLUSION These results may indicate that elevated oxidative stress (TBARS) is associated with weight gain after kidney Tx and that incorporating early clinical prevention strategies known to decrease oxidative stress could be recommended.

中文翻译：

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肾脏移植后12个月，氧化应激与接受者的体重增加有关。

目的肾移植（Tx）后体重增加被认为是不良结局的危险因素。氧化应激增加不仅与慢性肾脏疾病和Tx有关，而且与肥胖症和心血管疾病有关。这项初步研究的目的是测试肾脏Tx后12个月时氧化应激是否与体重增加有关，并初步了解涉及的潜在机制。设计与方法收件人（n = 33）分为两类：体重减轻和体重增加，基于它们在移植后12个月的体重变化。测量了总抗氧化剂容量（TAOC）和脂质过氧化（TBARS），以评估基线和12个月血浆中的氧化应激。进行了二次数据分析以鉴定潜在的基因调控。结果有17名收件人丢失（-6.63±5。52公斤），十六名接受者体重增加（8.94±6.18公斤）。与整个组的基线相比，在12个月时TAOC显着降低，但是，在两个时间点上，组之间都没有显着差异。在两个时间点，体重增加组中的TBARS均较高，在12个月时，TBARS显着较高（p = 0.012）。基因表达谱分析表明，在体重增加组中，脂肪组织中与活性氧相关基因相关的7个转录本在基线时表达显着降低，这可能是降低氧化应激的负反馈机制。结论这些结果可能表明氧化应激（TBARS）升高与肾脏Tx术后体重增加有关，因此建议采用已知的降低氧化应激的早期临床预防策略。16名接受者体重增加（8.94±6.18kg）。与整个组的基线相比，在12个月时TAOC显着降低，但是，在两个时间点上，组之间均无显着差异。在两个时间点，体重增加组中的TBARS均较高，在12个月时，TBARS显着较高（p = 0.012）。基因表达谱分析表明，在体重增加组中，脂肪组织中与活性氧相关基因相关的7个转录本在基线时表达显着降低，这可能是降低氧化应激的负反馈机制。结论这些结果可能表明氧化应激（TBARS）升高与肾脏Tx术后体重增加有关，因此建议采用已知的降低氧化应激的早期临床预防策略。16名接受者体重增加（8.94±6.18kg）。与整个组的基线相比，在12个月时TAOC显着降低，但是，在两个时间点上，组之间均无显着差异。在两个时间点，体重增加组中的TBARS均较高，在12个月时，TBARS显着较高（p = 0.012）。基因表达谱分析表明，在体重增加组中，脂肪组织中与活性氧相关基因相关的7个转录本在基线时表达显着降低，这可能是降低氧化应激的负反馈机制。结论这些结果可能表明氧化应激（TBARS）升高与肾脏Tx术后体重增加有关，因此建议采用已知的降低氧化应激的早期临床预防策略。