The pathway of CRISPR-Cas immunity redefines the roles of RNA in the flow of genetic information and ignites excitement for next-generation gene therapy tools. CRISPR-Cas machineries offer a fascinating set of new enzyme assemblies from which one can learn principles of molecular interactions and chemical activities. The interference step of the CRISPR-Cas immunity pathway congregates proteins, RNA, and DNA into a single molecular entity that selectively destroys invading nucleic acids. Although much remains to be discovered, a picture of how the interference process takes place is emerging. This review focuses on the current structural data for the three known types of RNA-guided nucleic acid interference mechanisms. In it, we describe key features of individual complexes and we emphasize comparisons across types and along functional stages. We aim to provide readers with a set of core principles learned from the three types of interference complexes and a deep appreciation of the diversity among them.

中文翻译：

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CRISPR-Cas监测和效应复合物的结构原理。

CRISPR-Cas免疫途径重新定义了RNA在遗传信息流中的作用，并激发了下一代基因治疗工具的兴奋性。CRISPR-Cas机器提供了一套引人入胜的新酶组合，从中人们可以学习分子相互作用和化学活性的原理。CRISPR-Cas免疫途径的干扰步骤将蛋白质，RNA和DNA聚合为一个分子实体，该分子实体选择性破坏了入侵的核酸。尽管仍有很多发现，但有关干扰过程如何发生的图片正在出现。这篇综述集中在三种已知类型的RNA引导的核酸干扰机制的当前结构数据。在其中，我们描述了单个复合体的关键特征，并强调了跨类型和沿功能阶段的比较。