Improvements in ex vivo generation of enucleated red blood cells are being sought for erythroid biology research, toward the ultimate goal of erythrocyte engineering for clinical use. Based upon the high levels of iron-saturated transferrin in plasma serum, it was hypothesized that terminal differentiation in serum-free media may be highly dependent on the concentration of iron. Here adult human CD34(+) cells were cultured in a serum-free medium containing dosed levels of iron-saturated transferrin (holo-Tf, 0.1-1.0 mg/ml). Iron in the culture medium was reduced, but not depleted, with erythroblast differentiation into haemoglobinized cells. At the lowest holo-Tf dose (0.1 mg/ml), terminal differentiation was significantly reduced and the majority of the cells underwent apoptotic death. Cell survival, differentiation and enucleation were enhanced as the holo-Tf dose increased. These data suggest that adequate holo-Tf dosing is critical for terminal differentiation and enucleation of human erythroblasts generated ex vivo in serum-free culture conditions. Published 2013. This article is a US Government work and is in the public domain in the USA.

中文翻译：

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无血清培养基中人成红细胞的铁剂量依赖性分化和去核。

红细胞生物学研究正在寻求在体外生成去核红细胞的改进，以实现临床使用的红细胞工程的最终目标。基于血浆血清中铁饱和转铁蛋白的高水平，假设无血清培养基中的终末分化可能高度依赖于铁的浓度。在这里，成人 CD34(+) 细胞在无血清培养基中培养，该培养基含有剂量水平的铁饱和转铁蛋白（holo-Tf，0.1-1.0 mg/ml）。随着成红细胞分化为血红蛋白化细胞，培养基中的铁减少，但并未耗尽。在最低 holo-Tf 剂量 (0.1 mg/ml) 下，终末分化显着降低，大多数细胞发生凋亡。细胞存活，随着holo-Tf剂量的增加，分化和去核增强。这些数据表明，足够的全息 Tf 剂量对于在无血清培养条件下离体产生的人类成红细胞的终末分化和去核至关重要。2013 年出版。本文是美国政府的作品，在美国属于公共领域。