A new series of organic-high ionic strength aqueous two-phase solvents systems was designed for separation of highly polar compounds by spiral high-speed counter-current chromatography. A total of 21 solvent systems composed of 1-butanol-ethanol-saturated ammonium sulfate-water at various volume ratios are arranged according to an increasing order of polarity. Selection of the two-phase solvent system for a single compound or a multiple sample mixture can be achieved by two steps of partition coefficient measurements using a graphic method. The capability of the method is demonstrated by optimization of partition coefficient for seven highly polar samples including tartrazine (K=0.77), tryptophan (K=1.00), methyl green (K= 0.93), tyrosine (0.81), metanephrine (K=0.89), tyramine (K=0.98), and normetanephrine (K=0.96). Three sulfonic acid components in D&C Green No. 8 were successfully separated by HSCCC using the graphic selection of the two-phase solvent system.

中文翻译：

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用于螺旋逆流色谱的有机高离子强度水性溶剂系统：分配系数的图形优化。

设计了一系列新的有机-高离子强度水性两相溶剂系统，用于通过螺旋高速逆流色谱分离高极性化合物。由不同体积比的1-丁醇-乙醇-饱和硫酸铵-水组成的共21个溶剂体系按照极性递增的顺序排列。可以通过使用图形方法的两个分配系数测量步骤来选择用于单个化合物或多个样品混合物的两相溶剂系统。该方法的能力通过优化七种高极性样品的分配系数来证明，包括柠檬黄 (K=0.77)、色氨酸 (K=1.00)、甲基绿 (K=0.93)、酪氨酸 (0.81)、变肾上腺素 (K=0.89) )、酪胺 (K=0.98) 和去甲变肾上腺素 (K=0.96)。