The enormous antigen receptor loci in lymphocytes are a paradigm of dynamic nuclear organisation, which is integral to their need to move extensively in 3D space to achieve distal gene synapse for V(D)J recombination and allelic exclusion. The loci undergo extensive 3D looping to bring distal genes together, controlled by several tissue-specific and ubiquitous factors, but how these factors achieve looping, synapsis and V(D)J recombination has been a mystery. Now several studies provide evidence that non-coding transcription, often proposed to play a role, is indeed an important driver, and furthermore has a specific nuclear destination for recombination. Both local transcription-independent looping and longer range factor-mediated transcription-dependent looping play separate roles in altering AgR architecture to enable V(D)J recombination.

中文翻译：

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免疫球蛋白重组的非编码转录和大规模核组织。

淋巴细胞中巨大的抗原受体基因座是动态核组织的范例，这对于它们需要在 3D 空间中广泛移动以实现 V(D)J 重组和等位基因排斥的远端基因突触是不可或缺的。基因座经历广泛的 3D 循环以将远端基因聚集在一起，由几个组织特异性和普遍存在的因素控制，但这些因素如何实现循环、突触和 V(D)J 重组一直是个谜。现在有几项研究提供了证据表明，通常被认为发挥作用的非编码转录确实是一个重要的驱动因素，而且还具有特定的重组核目的地。本地不依赖于转录的循环和较长范围的因子介导的依赖于转录的循环在改变 AgR 架构以启用 V(D)J 重组方面发挥着不同的作用。