当前位置: X-MOL 学术Contrast Media Mol. Imaging › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Rapid spectrophotometric technique for quantifying iron in cells labeled with superparamagnetic iron oxide nanoparticles: potential translation to the clinic.
Contrast Media & Molecular Imaging Pub Date : 2013-01-01 , DOI: 10.1002/cmmi.1493
Esmaeel R Dadashzadeh 1 , Matthew Hobson , L Henry Bryant , Dana D Dean , Joseph A Frank
Affiliation  

Labeling cells with superparamagnetic iron oxide (SPIO) nanoparticles provides the ability to track cells by magnetic resonance imaging. Quantifying intracellular iron concentration in SPIO labeled cells would allow for the comparison of agents and techniques used to magnetically label cells. Here we describe a rapid spectrophotometric technique (ST) to quantify iron content of SPIO-labeled cells, circumventing the previous requirement of an overnight acid digestion. Following lysis with 10% sodium dodecyl sulfate (SDS) of magnetically labeled cells, quantification of SPIO doped or labeled cells was performed using commonly available spectrophotometric instrument(s) by comparing absorptions at 370 and 750 nm with correction for turbidity of cellular products to determine the iron content of each sample. Standard curves demonstrated high linear correlation (R(2) = 0.998) between absorbance spectra of iron oxide nanoparticles and concentration in known SPIO-doped cells. Comparisons of the ST with inductively coupled plasma-mass spectroscopy (ICP-MS) or nuclear magnetic resonance relaxometric (R(2)) determinations of intracellular iron contents in SPIO containing samples resulted in significant linear correlation between the techniques (R(2) vs ST, R(2) > 0.992, p < 0.0001; ST vs ICP-MS, R(2) > 0.995, p < 0.0001) with the limit of detection of ST for iron = 0.66 µg ml(-1) for 10(6) cells ml(-1). We have developed a rapid straightforward protocol that does not require overnight acid digestion for quantifying iron oxide content in magnetically labeled cells using readily available analytic instrumentation that should greatly expedite advances in comparing SPIO agents and protocols for labeling cells.

中文翻译:


用于定量超顺磁性氧化铁纳米粒子标记的细胞中铁的快速分光光度技术:潜在的临床转化。



用超顺磁性氧化铁 (SPIO) 纳米粒子标记细胞提供了通过磁共振成像跟踪细胞的能力。量化 SPIO 标记细胞中的细胞内铁浓度将允许比较用于磁性标记细胞的试剂和技术。在这里,我们描述了一种快速分光光度技术(ST)来量化 SPIO 标记细胞的铁含量,绕过了之前过夜酸消化的要求。用 10% 十二烷基硫酸钠 (SDS) 裂解磁性标记的细胞后,使用常用分光光度仪器对 370 和 750 nm 处的吸光度进行比较,并校正细胞产物的浊度,从而对 SPIO 掺杂或标记的细胞进行定量。每个样品的铁含量。标准曲线表明氧化铁纳米粒子的吸收光谱与已知 SPIO 掺杂电池中的浓度之间存在高度线性相关性 (R(2) = 0.998)。 ST 与电感耦合等离子体质谱 (ICP-MS) 或核磁共振弛豫 (R(2)) 测定含 SPIO 样品中细胞内铁含量的比较结果表明,技术之间存在显着的线性相关性 (R(2) 与ST,R(2) > 0.992,p < 0.0001;ST 与 ICP-MS,R(2) > 0.995,p < 0.0001),铁的 ST 检测限 = 0.66 µg ml(-1) ) 对于 10(6) 个细胞 ml(-1)。我们开发了一种快速简单的方案,不需要过夜酸消化,即可使用现成的分析仪器定量磁性标记细胞中的氧化铁含量,这将大大加快比较 SPIO 试剂和标记细胞方案的进展。
更新日期:2019-11-01
down
wechat
bug