By using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (2:2:2:2, v/v/v/v), a high-speed counter-current chromatography technique was successfully used for isolation and purification of three alkaloids from Picrasma quassiodes (D. Don) Benn. for the first time. A total of 22.1 mg of 3-methylcanthin-2,6-dione, 4.9 mg of 4-methoxy-5-hydroxycanthin-6-one and 1.2 mg of 1-methoxycarbonyl-β-carboline were obtained from 100 mg of crude extract of Picrasma quassiodes (D. Don) Benn. in less than 5 hr, with purities of 89.30%, 98.32%, and 98.19%, respectively. The target compounds were identified by ESI-MS, 1H NMR, and 13C NMR.

中文翻译：

---

来自 PICRASMA QUASSIODES (D. DON) BENN 的生物碱的制备分离和纯化。通过高速逆流色谱法

采用正己烷-乙酸乙酯-甲醇-水（2:2:2:2，v/v/v/v）两相溶剂体系，成功应用高速逆流色谱技术用于从 Picrasma quassiodes (D. Don) Benn 中分离和纯化三种生物碱。首次。从 100 mg 的 3-methylcanthin-2,6-dione、4.9 mg 4-methoxy-5-hydroxycanthin-6-one 和 1.2 mg 1-甲氧基羰基-β-咔啉共获得 22.1 mg 3-methylcanthin-2,6-dione Picrasma quassiodes (D. Don) Benn。在不到 5 小时的时间内，纯度分别为 89.30%、98.32% 和 98.19%。目标化合物经 ESI-MS、1H NMR 和 13C NMR 鉴定。