BACKGROUND It is widely believed that laboratory strains of Escherichia coli, including those used for industrial production of proteins, do not secrete proteins to the extracellular milieu. RESULTS Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the autotransporter module. Yields were comparable to those achieved with other bacterial secretion systems. CONCLUSIONS The advantage of this module is that it relies on a relatively simple and easily manipulated secretion system, exhibits no apparent limitation to the size of the secreted protein and can deliver proteins to the extracellular environment at levels of purity and yields sufficient for many biotechnological applications.

中文翻译：

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用于重组蛋白选择性胞外积累的通用模块。

背景人们普遍认为，大肠杆菌的实验室菌株，包括那些用于工业生产蛋白质的菌株，不会将蛋白质分泌到细胞外环境中。结果 在这里，我们报告了基于大肠杆菌自转运体分泌系统的通用模块的开发，用于生产细胞外重组蛋白。我们证明，当与自转运体模块连接时，多种结构不同的蛋白质可以作为可溶性蛋白质分泌。产量与其他细菌分泌系统的产量相当。结论 该模块的优势在于它依赖于一个相对简单且易于操作的分泌系统，