Semi-preparative high-speed countercurrent chromatography (HSCCC) was successfully performed for isolation and purification of caffeic acid and 4 bioactive flavonoids from Eupatorium adenophorum Spreng using stepwise elution with a pair of two-phase solvent systems composed of ethyl acetate–methanol–water at the volume ratios of 10:1:10 and 5:1:5 (v/v). From 378.5 mg of crude extract 24.1 mg of caffeic acid, 6.7 mg of 4′-methyl quercetagetin 7-O-(6″-O-E-caffeoylglucopyranoside), 6.5 mg of quercetagetin 7-O-(6″-O-acetyl-β-D-glucopyranoside), 31.8 mg of eupalitin 3-O-β-D-galactopyranoside, and 36.7 mg of eupalitin were obtained with purities of 96.0%, 91.2%, 82.3%, 95.1%, and 85.6%, respectively. The structures of the separated compounds were identified by EI-MS, FAB-MS, 1HNMR, and 13CNMR.

中文翻译：

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使用高速逆流色谱逐步洗脱从紫茎泽兰中分离五种生物活性成分

半制备型高速逆流色谱 (HSCCC) 成功地分离纯化了紫茎泽兰中的咖啡酸和 4 种生物活性黄酮类化合物，使用由乙酸乙酯-甲醇-水组成的一对两相溶剂系统逐步洗脱10:1:10 和 5:1:5 (v/v) 的体积比。从 378.5 mg 粗提物中提取 24.1 mg 咖啡酸、6.7 mg 4'-甲基槲皮素 7-O-(6″-OE-咖啡酰吡喃葡萄糖苷)、6.5 mg 槲皮素 7-O-(6″-O-乙酰基-β) -D-吡喃葡萄糖苷)、31.8mg eupalitin 3-O-β-D-吡喃半乳糖苷和 36.7mg eupaltin，分别具有 96.0%、91.2%、82.3%、95.1% 和 85.6% 的纯度。分离化合物的结构通过EI-MS、FAB-MS、1HNMR和13CNMR进行鉴定。