Single-cell microscopy studies have the potential to provide an unprecedented view of gene expression with exquisite spatial and temporal sensitivity. However, there is a challenge to connect the holistic cellular view with a reductionist biochemical view. In particular, experimental efforts to characterize the in vivo regulation of transcription have focused primarily on measurements of the dynamics of transcription factors and chromatin modifying factors. Such measurements have elucidated the transient nature of many nuclear interactions. In the past few years, experimental approaches have emerged that allow for interrogation of the output of transcription at the single-molecule, single-cell level. Here, I summarize the experimental results and models that aim to provide an integrated view of transcriptional regulation.

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关于转录机制，表达动力学告诉我们什么？

单细胞显微镜研究有可能提供前所未有的基因表达视图，并具有敏锐的空间和时间敏感性。然而，将整体细胞观点与还原论生化观点联系起来是一个挑战。特别是，表征体内转录调控的实验工作主要集中在测量转录因子和染色质修饰因子的动力学上。此类测量阐明了许多核相互作用的瞬态性质。在过去几年中，出现了允许在单分子、单细胞水平上询问转录输出的实验方法。在这里，我总结了旨在提供转录调控综合视图的实验结果和模型。