Abstract High-speed countercurrent chromatography (HSCCC) was successfully applied for the first time for the isolation and purification of psoralen and bergapten from the plant Ficus carica L leaves. The crude extract was obtained by extraction with light petroleum (b.p.: 60°C–90°C) from the dried leaves of Ficus carica L. n-Hexane-ethyl acetate-methanol-water (1:1:1:1, v/v) was used as the two-phase solvent system. Each peak fraction was analyzed by high performance liquid chromatography (HPLC). The method yielded 4.4 mg of psoralen at 99.1% purity and 2.1 mg of bergapten at 98.2% purity from 400 mg of the crude extract in a single run. The structures of the two compounds were identified by 1H-NMR, 13C-NMR, and MS.

中文翻译：

---

从榕树中分离和纯化补骨脂素和佛手柑。叶用高速逆流色谱法

摘要 高速逆流色谱法（HSCCC）首次成功应用于植物榕树叶中补骨脂素和佛手柑的分离纯化。粗提物是用石油醚 (bp: 60°C–90°C) 从榕树的干叶中提取得到的。 正己烷-乙酸乙酯-甲醇-水 (1:1:1:1, v /v) 用作两相溶剂系统。通过高效液相色谱（HPLC）分析每个峰级分。该方法在单次运行中从 400 mg 粗提取物中得到 4.4 mg 纯度为 99.1% 的补骨脂素和 2.1 mg 纯度为 98.2% 的佛手柑。两种化合物的结构经1H-NMR、13C-NMR和MS鉴定。