Abstract Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800 rpm. This yielded 72 mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR.

中文翻译：

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制备型高速逆流色谱法分离沙乌飞燕草中的马来酸


摘要 采用高速逆流色谱（HSCCC）首次实现了飞燕草中的弹性蛋白的制备分离。以乙酸乙酯-氯仿-甲醇-水(3:0.1:2:3,v/v)组成的溶剂体系，下层有机相为流动相，转速800rpm进行分离。得到 72 毫克的弹性蛋白，纯度超过 97%，回收率约为 95%。通过MS和NMR鉴定了化学结构。