The endomembrane system of eukaryotic cells uses membrane-enclosed carriers to move diverse macromolecules among different membrane-bound compartments, a requirement for cells to secrete and take up molecules from their environment. Two recycling pathways-biosynthetic and endocytic, each with specific lipid components-make up this system, with the Golgi apparatus mediating transport between the two. Here, we integrate lipid-based mechanisms into the description of this system. A partitioning model of the Golgi apparatus is discussed as a working hypothesis to explain how membrane lipids and proteins that are segregated based on lateral lipid partitioning support the unique composition of the biosynthetic and endocytic recycling pathways in the face of constant trafficking of molecular constituents. We further discuss how computational modeling can allow for interpretation of experimental findings and provide mechanistic insight into these important cellular pathways.

中文翻译：

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脂质和胆固醇作为内膜系统交通的调节剂。


真核细胞的内膜系统使用膜封闭载体在不同的膜结合区室之间移动不同的大分子，这是细胞从环境中分泌和吸收分子的要求。两种循环途径——生物合成和内吞，每种途径都具有特定的脂质成分——构成了这个系统，高尔基体介导两者之间的运输。在这里，我们将基于脂质的机制整合到该系统的描述中。高尔基体的分配模型被讨论为一个工作假设，以解释基于横向脂质分配而分离的膜脂和蛋白质如何在面对分子成分的不断运输时支持生物合成和内吞再循环途径的独特组成。我们进一步讨论计算模型如何解释实验结果并提供对这些重要细胞途径的机制洞察。