Combined with medium pressure liquid chromatography (MPLC) and preparative high performance liquid chromatography (perp-HPLC), high speed countercurrent chromatography (HSCCC) was applied for the separation and purification of flavone C-glycosides from the crude extract of leaves of Ficus microcarpa L.f. HSCCC separation was performed on a two-phase solvent system composed of methyl tert- butyl ether–ethyl acetate –1-butanol–acetonitrile–0.1% aqueous trifluoroacetic acid at a volume ratio of 1:3:1:1:5. Partially resolved peak fractions from HSCCC separation were further purified by preparative HPLC. Four well separated compounds were obtained and their purities were determined by HPLC. The purities of these peaks were 97.28%, 97.20%, 92.23%, and 98.40%. These peaks were characterized by ESI-MSn. According to the reference, they were identified as orientin (peak I), isovitexin-3″-O-glucopyranoside (peak II), isovitexin (peak III), and vitexin (peak IV), and yielded 1.2 mg, 4.5 mg, 3.3 mg, and 1.8 mg, respectively.

中文翻译：

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通过中压液相色谱、高速逆流色谱和制备液相色谱法分离和纯化小叶中的黄酮 C-糖苷。

结合中压液相色谱 (MPLC) 和制备型高效液相色谱 (perp-HPLC)，应用高速逆流色谱 (HSCCC) 分离纯化小果榕 (Ficus microcarpa Lf) 叶粗提物中的黄酮 C-苷HSCCC 分离在由甲基叔丁基醚-乙酸乙酯-1-丁醇-乙腈-0.1% 三氟乙酸水溶液组成的两相溶剂系统上进行，体积比为 1:3:1:1:5。从 HSCCC 分离中部分分离的峰级分通过制备型 HPLC 进一步纯化。获得了四种分离良好的化合物，并通过 HPLC 测定了它们的纯度。这些峰的纯度分别为 97.28%、97.20%、92.23% 和 98.40%。这些峰由 ESI-MSn 表征。根据参考资料，