Abstract Preparative high speed countercurrent chromatography (HSCCC) has been successfully used for the isolation and purification of isorhamnetin from Stigma maydis. This was achieved in two stages: the first separation was performed with a two phase solvent system composed of n-hexane-ethyl acetate-methanol-water (HEMW) at a volume ratio of 5:5:5:5, yielding isorhamnetin at 65.6%, which is followed by the second run using a two phase solvent system composed of HEMW 5:5:6:4, v/v. From 700 mg of the crude extract, 11.8 mg of isorhamnetin was obtained at a high purity of 98%. The final identification was performed by MS, 1H-NMR and 13C-NMR spectra.

中文翻译：

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采用高速逆流色谱法从玉米须中制备分离异鼠李素


摘要 制备型高速逆流色谱（HSCCC）已成功用于从玉米须中分离纯化异鼠李素。这是通过两个阶段实现的：第一次分离采用由体积比为 5:5:5:5 的正己烷-乙酸乙酯-甲醇-水 (HEMW) 组成的两相溶剂系统进行，异鼠李素产量为 65.6 %，然后使用由 HEMW 5:5:6:4（v/v）组成的两相溶剂系统进行第二次运行。从700mg粗提物中得到11.8mg异鼠李素，纯度高达98%。最终通过MS、1H-NMR和13C-NMR谱进行鉴定。