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Preparative Isolation of Isorhamnetin fromStigma Maydisusing High Speed Countercurrent Chromatography
Journal of Liquid Chromatography & Related Technologies ( IF 1.0 ) Pub Date : 2008-12-30 , DOI: 10.1080/10826070802603369
Xiaoying Cao 1 , Yun Wei , Yoichiro Ito
Affiliation  

Abstract Preparative high speed countercurrent chromatography (HSCCC) has been successfully used for the isolation and purification of isorhamnetin from Stigma maydis. This was achieved in two stages: the first separation was performed with a two phase solvent system composed of n-hexane-ethyl acetate-methanol-water (HEMW) at a volume ratio of 5:5:5:5, yielding isorhamnetin at 65.6%, which is followed by the second run using a two phase solvent system composed of HEMW 5:5:6:4, v/v. From 700 mg of the crude extract, 11.8 mg of isorhamnetin was obtained at a high purity of 98%. The final identification was performed by MS, 1H-NMR and 13C-NMR spectra.

中文翻译:


采用高速逆流色谱法从玉米须中制备分离异鼠李素



摘要 制备型高速逆流色谱(HSCCC)已成功用于从玉米须中分离纯化异鼠李素。这是通过两个阶段实现的:第一次分离采用由体积比为 5:5:5:5 的正己烷-乙酸乙酯-甲醇-水 (HEMW) 组成的两相溶剂系统进行,异鼠李素产量为 65.6 %,然后使用由 HEMW 5:5:6:4(v/v)组成的两相溶剂系统进行第二次运行。从700mg粗提物中得到11.8mg异鼠李素,纯度高达98%。最终通过MS、1H-NMR和13C-NMR谱进行鉴定。
更新日期:2008-12-30
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