Abstract Cordycepin is successfully isolated and purified from Cordyceps millitaris in a two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate–1-butanol–water (3:2:5, v/v/v) and trichloromethane–methanol–1-butanol–water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatographic analysis.

中文翻译：

---

高速逆流色谱法两步纯化虫草菌中的虫草素

摘要 采用高速逆流色谱法通过两步纯化方法成功地从虫草中分离纯化虫草素。两种溶剂系统，乙酸乙酯-1-丁醇-水（3:2:5，v/v/v）和三氯甲烷-甲醇-1-丁醇-水（2:1:0.25:1，v/v/v/ v)，用于两步纯化。制备的虫草素经高效液相色谱分析纯度为98.1%。