Abstract Chickpea is known as a plant that is rich in protein, carbohydrates, and nutrition, and its seeds and sprouts have been processed into various health foods. In the present study, four isoflavones were purified from the seeds and sprouts of chickpea by high-speed countercurrent chromatography (HSCCC) using two biphasic solvent systems composed of n-hexane–ethyl acetate–methanol–water (5:5:5:5, v/v) and ethyl acetate–water (1:1, v/v). The results indicated that 14.2 mg of formononetin, 15.7 mg of biochanin A, 9.1 mg of ononin, 11.3 mg of biochanin A-7-O-β-D-glucoside were obtained from 150 mg of sprout extracts with the purity of 92.26%, 95.86%, 95.32%, and 96.56%, respectively. Compared with the sprouts, separation of seed extracts yielded less amounts of biochanin A-7-O-β-D-glucoside and biochanin A with lower purity. The results indicate that four main isoflavones in chickpea, i.e., isoflavones, formononetin, biochanin A, ononin, and biochanin A-7-O-β-D-glucoside, are substantially increased by biosynthesis during the seed germination.

中文翻译：

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高速逆流色谱法分离纯化鹰嘴豆种子和豆芽中异黄酮的比较研究


摘要 鹰嘴豆被誉为富含蛋白质、碳水化合物和营养的植物，其种子和豆芽已被加工成各种保健食品。在本研究中，使用由正己烷-乙酸乙酯-甲醇-水（5:5:5:5）组成的两种双相溶剂系统，通过高速逆流色谱（HSCCC）从鹰嘴豆种子和豆芽中纯化出四种异黄酮。 ，v/v）和乙酸乙酯-水（1:1，v/v）。结果表明，从150 mg豆芽提取物中可得芒柄花素14.2 mg、生物鸡素A 15.7 mg、灯盏花素9.1 mg、生物鸡素A-7-O-β-D-葡萄糖苷11.3 mg，纯度为92.26%。分别为 95.86%、95.32% 和 96.56%。与豆芽相比，种子提取物分离得到的生物鸡素A-7-O-β-D-葡萄糖苷含量较少，生物鸡素A纯度也较低。结果表明，鹰嘴豆中的四种主要异黄酮，即异黄酮、芒柄花素、生物鸡精A、洋葱素和生物鸡精A-7-O-β-D-葡萄糖苷，在种子萌发过程中通过生物合成显着增加。