Abstract High speed countercurrent chromatography (HSCCC) was successfully used to isolate three bioactive compounds, i.e., amygdalin from bitter almond and taxifolin-3-glucoside and quercetin-3-galactoside (hyperoside) from water extract of A. pilosa Ledeb, respectively. From 1 g of the crude extract 65 mg of amygdalin was isolated at 97% purity using a two phase solvent system composed of ethyl acetate-n-butanol-water (5:2:5, v/v) by preparative HSCCC. From a 400 mg amount of crude extract of A. pilosa Ledeb, 11 mg of taxifolin-3-glucoside and 8 mg of hyperoside were isolated at 96% purity, using a two phase solvent system composed of ethyl acetate-methanol-water (25:1:25, v/v) similarly by preparative HSCCC. The final structural identification was performed by MS, 1H-NMR, and 13C-NMR spectra.

中文翻译：

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高速逆流色谱法从植物提取物中制备 Axifolin-3-Glucoside、金丝桃苷和苦杏仁苷的分离

摘要 采用高速逆流色谱法(HSCCC) 成功分离出苦杏仁中的苦杏仁苷、紫杉水提物中的紫杉叶素-3-葡萄糖苷和槲皮素-3-半乳糖苷(金丝桃苷) 三种生物活性化合物。使用由乙酸乙酯-正丁醇-水 (5:2:5, v/v) 组成的两相溶剂系统，通过制备型 HSCCC 从 1 g 的粗提取物中分离出 65 mg 纯度为 97% 的苦杏仁苷。使用由乙酸乙酯-甲醇-水 (25 :1:25, v/v) 同样通过制备型 HSCCC。最终的结构鉴定通过 MS、1H-NMR 和 13C-NMR 光谱进行。