Abstract A novel spiral tube support is introduced which allows accommodating a multlilayer spiral column made of a long piece of fluorinated plastic tubing. Two spiral columns tested in this study consist of 1.6 mm ID and 0.85 mm ID FEP (fluorinated ethylenepropylene) and PTFE (polytetrafluoroethylene) tubing with the total capacity of 100 mL and 40 mL, respectively. Performance of these spiral columns was examined with a two phase solvent system composed of 1‐butanol/acetic acid/water at a volume ratio of 4∶1∶5 using tryptophyl‐tyrosine (try‐tyr) and valyl‐tyrosine (val‐tyr) as test samples under various revolution speeds and flow rates. Among 4 different elution modes tested, L‐I‐T (lower phase pumped from the internal head end of the spiral column) and U‐O‐H (upper phase pumped from the external tail of the spiral column) at a low flow rate produced the best results especially for both columns. The highest peak resolution (Rs) of over 3.5 was obtained from U‐O‐H at a flow rate of 1 mL/min with 74% stationary phase retention. At a flow rate of 5 mL/min, the highest revolution speed at 1,200 rpm improved the peak resolution in both elution modes. The present system may be useful for purification of various polar compounds in biomedical researches.

中文翻译：

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用于高速逆流色谱的螺旋管支架


摘要 介绍了一种新型螺旋管支架，可容纳由长段氟化塑料管制成的多层螺旋柱。本研究中测试的两个螺旋柱由内径 1.6 毫米和内径 0.85 毫米的 FEP（氟化乙烯丙烯）和 PTFE（聚四氟乙烯）管组成，总容量分别为 100 mL 和 40 mL。使用色氨酸-酪氨酸（try-tyr）和缬氨酰-酪氨酸（val-tyr），使用体积比为 4:1:5 的 1-丁醇/乙酸/水组成的两相溶剂系统，检查了这些螺旋柱的性能。 ）作为各种转速和流量下的测试样品。在测试的 4 种不同洗脱模式中，低流速下的 L-I-T（从螺旋柱内部头端泵送下层相）和 U-O-H（从螺旋柱外部尾部泵送上层相）产生了最好的结果，尤其是对于两根色谱柱。 U-O-H 在 1 mL/min 的流速下获得超过 3.5 的最高峰分辨率 (Rs)，固定相保留率为 74%。在 5 mL/min 流速下，1,200 rpm 的最高转速提高了两种洗脱模式下的峰分辨率。本系统可用于生物医学研究中各种极性化合物的纯化。