Biochemical networks are characterized by recurrent patterns and motifs, but the design principles underlying the dynamics of the mammalian intracellular signalling network remain unclear. We systematically analysed decay rates of 134 signalling proteins and investigated their gene expression profiles in response to stimulation to get insights into transcriptional feedback regulation. We found a clear separation of the signalling pathways into flexible and static parts: for each pathway a subgroup of unstable signal inhibitors is transcriptionally induced upon stimulation, while the other constitutively expressed signalling proteins are long-lived. Kinetic modelling suggests that this design principle allows for swift feedback regulation and establishes latency phases after signalling, and that it might be an optimal design due to a trade-off between energy efficiency and flexibility.

中文翻译：

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哺乳动物信号网络反馈调节中的循环设计模式。

生化网络的特点是反复出现的模式和图案，但哺乳动物细胞内信号网络动力学的设计原则仍不清楚。我们系统地分析了 134 种信号蛋白的衰减率，并研究了它们响应刺激的基因表达谱，以深入了解转录反馈调节。我们发现信号通路清楚地分为灵活和静态部分：对于每个通路，在刺激后转录诱导了不稳定信号抑制剂的亚组，而其他组成型表达的信号蛋白则是长寿命的。动力学建模表明，这种设计原则允许快速反馈调节并在发出信号后建立延迟阶段，