Gold nanoparticles (AuNPs) as the most excellent anticancer theranostic nanoparticles were synthesized through efficient, simple, and green synthesis method using Marsdenia tenacissima plant extracts and they are widely characterized by several techniques including ultraviolet-visible (UV) spectroscopy, atomic force microscopy (AFM), energy-dispersive X-ray spectrometers (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) spectroscopy. From the AuNPs synthesized by M. tenacissima extracts, it was discovered that particle size around 50 nm, which is admirable nano dimension, was achieved by plant-mediated synthesis. After characterization of these nanoparticles, they performed as in vitro anticancer activity against lung cancer cell lines (A549). MTT assay revealed that AuNPs produce toxicity based on the dose-dependent A549 cells growth inhibition. AuNPs treatment activates caspase expression and down-regulates the anti-apoptotic protein expression in A549 cells. Our results point out that the AuNPs from M. tenacissima extract are apposite stabilizing agents, which serve as an effective anticancer agent against lung cancer cell lines (A549).

中文翻译：

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拟南芥（Marsdenia tenacissima）的绿色合成金纳米颗粒的抗癌活性可通过凋亡途径抑制A549细胞增殖。

金纳米颗粒（AuNPs）是最优秀的抗癌治疗药物纳米颗粒，是通过使用马氏蕨（Marsdenia tenacissima）植物提取物通过高效，简单且绿色的合成方法合成的，并通过多种技术进行了广泛表征，包括紫外可见光谱（UV），原子力显微镜（AFM） ），能量色散X射线光谱仪（EDS），透射电子显微镜（TEM）和傅里叶变换红外（FT-IR）光谱。从tenacissima提取物合成的AuNPs中，发现通过植物介导的合成可以达到约50 nm的纳米级尺寸。对这些纳米颗粒进行表征后，它们表现出了针对肺癌细胞系（A549）的体外抗癌活性。MTT分析显示，AuNPs基于剂量依赖性A549细胞生长抑制产生毒性。AuNPs处理可激活caspase表达并下调A549细胞中的抗凋亡蛋白表达。我们的结果指出，来自tenacissima提取物的AuNPs是合适的稳定剂，可作为针对肺癌细胞系（A549）的有效抗癌剂。