Adipose derived stem cells (ADSCs) are multipotent and can transdifferentiate into neural stem cells. We investigated the transdifferentiation of ADSCs to neural phenotype (NP) cells using selegiline and two-dimensional electrophoresis (2-DE). The perinephric and inguinal fat of rats was collected and used to isolate ADSCs that were characterized by immunophenotyping using flow cytometry. The ADSCs were differentiated into osteogenic and lipogenic cells. The NP cells were generated using 10-9 mM selegiline and characterized by immunocytochemical staining of nestin and neurofilament 68 (NF-68), and by qRT-PCR of nestin, neurod1 and NF68. Total protein of ADSCs and NP cells was extracted and their proteome patterns were examined using 2-DE. ADSCs carried CD73, CD44 and CD90 cell markers, but not CD34. ADSCs were differentiated into osteocyte and adipocyte lineages. The differentiated NP cells expressed nestin, neuro d1 and NF-68. The proteome pattern of ADSCs was compared with that of NP cells and eight spots showed more than a two fold increase in protein expression. The molecular weights and isoelectric points of these highly expressed proteins were estimated using Melanie software. We compared these results with those of the mouse proteomic database using the protein isoelectric point database, and the functions of the eight proteins in differentiation of NP cells were predicted using the UniProt database. The probable identities of the proteins that showed higher expression in NP cells included cholinesterase, GFRa2, protein kinase C (PKC-eta) and RING finger protein 121. The sequences of the proteins identified from mouse database were aligned by comparing them with similar proteins in rat database using the Basic Local Alignment Search Tool (BLAST). The E values of all aligned proteins were zero, which indicates consistency of the matched protein. These proteins participate in differentiation of the neuron and their overexpression causes ADSCs transdifferentiation into NP cells.

中文翻译：

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使用二维凝胶电泳分析比较脂肪干细胞与司来吉兰处理后的蛋白质组模式。

脂肪来源的干细胞（ADSC）是多能的，可以转分化为神经干细胞。我们使用司来吉兰和二维电泳（2-DE）研究了ADSCs向神经表型（NP）细胞的转分化。收集大鼠的肾上腺和腹股沟脂肪，并用于分离通过流式细胞仪进行免疫表型鉴定的ADSC。ADSCs分化为成骨和脂肪细胞。NP细胞使用10-9 mM司来吉兰产生，并通过巢蛋白和神经丝68（NF-68）的免疫细胞化学染色，以及巢蛋白，neurod1和NF68的qRT-PCR进行表征。提取ADSC和NP细胞的总蛋白，并使用2-DE检查其蛋白质组模式。ADSC携带CD73，CD44和CD90细胞标记物，但不携带CD34。ADSCs分为骨细胞和脂肪细胞谱系。分化的NP细胞表达巢蛋白，神经d1和NF-68。将ADSCs的蛋白质组模式与NP细胞的蛋白质组模式进行比较，八个斑点显示蛋白质表达增加了两倍以上。使用Melanie软件估算这些高表达蛋白的分子量和等电点。我们将这些结果与使用蛋白质等电点数据库的小鼠蛋白质组学数据库的结果进行了比较，并使用UniProt数据库预测了这8种蛋白质在NP细胞分化中的功能。在NP细胞中显示较高表达的蛋白质的可能身份包括胆碱酯酶，GFRa2，蛋白激酶C（PKC-eta）和RING指蛋白121。通过使用基本局部比对搜索工具（BLAST）将它们与大鼠数据库中的相似蛋白质进行比较，比对从小鼠数据库中鉴定出的蛋白质序列。所有比对的蛋白质的E值均为零，表明匹配的蛋白质的一致性。这些蛋白质参与神经元的分化，它们的过度表达导致ADSC转分化为NP细胞。