Objective: This research was aimed to detect the functions of Lycium barbarum polysaccharides (LBPs) on oxygen and glucose deprivation (OGD) injury and potential mechanisms at PC-12 cells. Methods: CCK-8, flow cytometry and reactive oxygen species (ROS) assays were used to detect OGD, LBPs and miR-24 effects on cell viability, apoptosis, and oxidative stress. MiR-24 was transfected and texted by transfection and qRT-PCR. Moreover, the related-protein levels of apoptosis, autophagy and pathways were tested by Western blotting. Results: LBPs significantly enhanced cell viability , inhibited cell apoptosis, autophagy and ROS level in OGD injury. In addition, miR-24 expression was declined in OGD-treated cells, while it was elevated when added LBPs. The preventive effects of LBPs on PC-12 cell damage induced by OGD were reversed by down-regulating miR-24. Furthermore, miR-24 inhibitor declined LBPs-induced change in Wnt/β-catenin and JAK1/STAT3 pathways in OGD-injuried cells. Conclusions: LBPs exhibited preventive effects via up-regulating miR-122 and activating Wnt/β-catenin and JAK1/STAT3 pathways in OGD-induced PC-12 cells.

中文翻译：

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枸杞多糖通过上调miR-24减轻氧葡萄糖剥夺诱导的PC-12细胞损伤。

目的：本研究旨在检测枸杞多糖（LBPs）对氧和葡萄糖剥夺（OGD）损伤的功能以及PC-12细胞的潜在机制。方法：使用CCK-8，流式细胞仪和活性氧（ROS）分析检测OGD，LBP和miR-24对细胞活力，细胞凋亡和氧化应激的影响。将MiR-24转染并通过转染和qRT-PCR进行文本分析。此外，通过蛋白质印迹法检测凋亡，自噬和途径的相关蛋白水平。结果：在OGD损伤中，LBP显着增强了细胞活力，抑制了细胞凋亡，自噬和ROS水平。另外，在经OGD处理的细胞中，miR-24表达下降，而在添加LBP时，miR-24表达升高。下调miR-24可以逆转LBP对OGD诱导的PC-12细胞损伤的预防作用。此外，miR-24抑制剂可降低LBP诱导的OGD损伤细胞中Wnt /β-catenin和JAK1 / STAT3途径的变化。结论：LBP通过上调miR-122并激活OGD诱导的PC-12细胞中的Wnt /β-catenin和JAK1 / STAT3途径发挥预防作用。