Ion-complementary self-assembling peptides have potential in delivering hydrophobic drugs. This study involved two self-assembling peptides, RADA16-I and RVDV16-I, of which RVDV16-I was a novel self-assembling peptide with different hydrophobic side chains designed from RADA16-I. The purpose of this study was to observe the interaction between different self-assembling peptides and emodin through fluorescence spectrophotometry, CD, SEM and AFM; to construct a preliminary suspension in-situ hydrogel delivery system for emodin with the self-assembling peptides; and to investigate the drug-loading and drug-releasing properties of the self-assembling peptides on emodin. The results showed that both peptides can interact with emodin and the interaction was dominated by hydrophobic interaction. The aqueous solutions of both self-assembling peptides can form relatively stable suspensions with emodin under mechanical stirring, and the suspension can form in-situ hydrogel under physiological condition. In vitro release of emodin from the hydrogels showed a manner of sustained release to some extent. Cell viability studies showed inherent proliferation inhibiting effects of emodin on tumor cells was maintained or enhanced through the in-situ hydrogels. The self-assembling peptides RADA16-I and RVDV16-I had showed promising drug-loading and drug-releasing performance for hydrophobic drugs. It is reasonable to exploit self-assembling peptides as drug carriers for their great potential to improve delivery of hydrophobic drugs.

中文翻译：

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自组装肽与大黄素之间的相互作用以及大黄素从原位水凝胶中的受控释放。

离子互补的自组装肽具有输送疏水性药物的潜力。这项研究涉及两个自组装肽RADA16-I和RVDV16-I，其中RVDV16-I是一种新颖的自组装肽，具有从RADA16-I设计的不同疏水侧链。本研究的目的是通过荧光分光光度法，CD，SEM和AFM观察不同自组装肽与大黄素之间的相互作用。用自组装肽构建大黄素的初步悬浮原位水凝胶输送系统；并研究大黄素上自组装肽的载药量和释药性能。结果表明，两种肽均可与大黄素相互作用，且相互作用以疏水相互作用为主。两种自组装肽的水溶液可以在机械搅拌下与大黄素形成相对稳定的悬浮液，并且该悬浮液在生理条件下可以原位形成水凝胶。大黄素在体外从水凝胶中的释放显示出一定程度的持续释放方式。细胞活力研究表明，大黄素对肿瘤细胞的内在增殖抑制作用通过原位水凝胶得以维持或增强。自组装肽RADA16-I和RVDV16-I对疏水性药物显示出有希望的载药量和释放药物的性能。利用自组装肽作为药物载体是合理的，因为它们具有改善疏水性药物递送的巨大潜力。悬浮液可在生理条件下原位形成水凝胶。大黄素在体外从水凝胶中的释放显示出一定程度的持续释放方式。细胞活力研究表明，大黄素对肿瘤细胞的内在增殖抑制作用通过原位水凝胶得以维持或增强。自组装肽RADA16-I和RVDV16-I对疏水性药物显示出有希望的载药量和释放药物的性能。利用自组装肽作为药物载体是合理的，因为它们具有改善疏水性药物递送的巨大潜力。悬浮液可在生理条件下原位形成水凝胶。大黄素在体外从水凝胶中的释放显示出一定程度的持续释放方式。细胞活力研究表明，大黄素对肿瘤细胞的内在增殖抑制作用通过原位水凝胶得以维持或增强。自组装肽RADA16-I和RVDV16-I对疏水性药物显示出有希望的载药量和释放药物的性能。利用自组装肽作为药物载体是合理的，因为它们具有改善疏水性药物递送的巨大潜力。细胞活力研究表明，大黄素对肿瘤细胞的内在增殖抑制作用通过原位水凝胶得以维持或增强。自组装肽RADA16-I和RVDV16-I对疏水性药物显示出有希望的载药量和释放药物的性能。利用自组装肽作为药物载体是合理的，因为它们具有改善疏水性药物递送的巨大潜力。细胞活力研究表明，大黄素对肿瘤细胞的内在增殖抑制作用通过原位水凝胶得以维持或增强。自组装肽RADA16-I和RVDV16-I对疏水性药物显示出有希望的载药量和药物释放性能。利用自组装肽作为药物载体是合理的，因为它们具有改善疏水性药物递送的巨大潜力。