The alterations of the intestinal proteome were observed in intrauterine growth restriction (IUGR) piglets during early life by gel-based approaches. Nevertheless, how IUGR affects the intestinal membrane proteome during neonatal development remains unclear. Here, we applied the iTRAQ-based proteomics technology and biochemical analysis to investigate the impact of IUGR on the membrane proteome of the jejunal mucosa in the piglets. Three hundred sixty-one membrane proteins were screened by functional prediction. Among them, eight, five, and one differentially expressed membrane proteins were identified between IUGR and NBW piglets at day 0, day 7, and day 21 after birth, respectively. Differentially expressed membrane proteins (DEMPs) including F1SBL3, F1RRW8, F1S539, F1S2Z2, F1RIR2, F1RUF2 I3LP60, Q2EN79, and F1SIH8 were reduced while the relative abundance of I3L6A2, F1SCJ1, F1RI18, I3LRJ7, and F1RNN0 were increased in IUGR piglets than NBW piglets. From the aspects of function, F1RRW8, F1S539, F1S2Z2, and F1RIR2 are mainly associated with D2 dopamine receptor binding, transmembrane transport of small molecules, signal transduction, and translocation of GLUT4, respectively, and F1SIH8, I3LRJ7, and F1RNN0 are related to autophagy, metabolism of vitamins, and intracellular protein transport. Additionally, IUGR decreased the level of proteins (F1RRW8, Q2EN79, and F1RI18) that are involved in response to oxidative stress.

中文翻译：

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膜蛋白质组学分析显示，仔猪的子宫内生长受限会破坏肠道发育。

通过基于凝胶的方法，在早期的宫内生长受限（IUGR）仔猪中观察到肠道蛋白质组的变化。尽管如此，IUGR如何影响新生儿发育过程中的肠膜蛋白质组仍不清楚。在这里，我们应用了基于iTRAQ的蛋白质组学技术和生化分析来研究IUGR对仔猪空肠黏膜膜蛋白质组的影响。通过功能预测筛选了361个膜蛋白。其中，分别在出生后第0天，第7天和第21天在IUGR和NBW仔猪之间鉴定出八种，五种和一种差异表达的膜蛋白。差异表达的膜蛋白（DEMP），包括F1SBL3，F1RRW8，F1S539，F1S2Z2，F1RIR2，F1RUF2 I3LP60，Q2EN79，与NBW仔猪相比，IUGR仔猪的I3L6A2，F1SCJ1，F1RI18，I3LRJ7和F1RNN0的相对丰度增加，而F1SIH8减少。从功能方面来看，F1RRW8，F1S539，F1S2Z2和F1RIR2主要与D2多巴胺受体结合，小分子的跨膜转运，GLUT4的信号转导和易位有关，而F1SIH8，I3LRJ7和F1RNN0与自噬有关，维生素的代谢和细胞内蛋白质的运输。此外，IUGR降低了响应氧化应激的蛋白质（F1RRW8，Q2EN79和F1RI18）的水平。小分子的跨膜转运，GLUT4的信号转导和转运以及F1SIH8，I3LRJ7和F1RNN0与自噬，维生素的代谢和细胞内蛋白质的转运有关。此外，IUGR降低了响应氧化应激的蛋白质（F1RRW8，Q2EN79和F1RI18）的水平。小分子的跨膜转运，GLUT4的信号转导和转运以及F1SIH8，I3LRJ7和F1RNN0与自噬，维生素的代谢和细胞内蛋白质的转运有关。此外，IUGR降低了响应氧化应激的蛋白质（F1RRW8，Q2EN79和F1RI18）的水平。