Recent advances in structure determination of sub-cellular structures using cryo-electron microscopy and tomography have enabled us to understand their architecture in a more detailed manner and gain insight into their function. The choice of approach to use for atomic model building, fitting, refinement and validation in the 3D map resulting from these experiments depends primarily on the resolution of the map and the prior information on the corresponding model. Here, we survey some of such methods and approaches and highlight their uses in specific recent examples.

中文翻译：

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冷冻电镜图中的结构建模。

使用冷冻电子显微镜和断层扫描技术确定亚细胞结构的最新进展使我们能够更详细地了解它们的结构并深入了解它们的功能。在这些实验产生的 3D 地图中选择用于原子模型构建、拟合、细化和验证的方法主要取决于地图的分辨率和相应模型的先验信息。在这里，我们调查了其中一些方法和途径，并重点介绍了它们在最近的具体示例中的用途。