Electron microscopy imaging of post-mortem human brain (PMHB) comes with a unique set of challenges due to numerous parameters beyond the researcher's control. Nevertheless, the wealth of information provided by the ultrastructural analysis of PMHB is proving crucial in our understanding of neurodegenerative diseases. This review highlights the importance of such studies and covers challenges, limitations and recent developments in the application of current EM imaging, including cryo-ET and correlative hybrid techniques, on PMHB.

中文翻译：

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使用电子和X射线显微镜对死后人脑组织进行成像。

由于研究人员无法控制的众多参数，验尸后人脑（PMHB）的电子显微镜成像面临一系列独特的挑战。然而，事实证明，由PMHB的超微结构分析提供的大量信息对于我们对神经退行性疾病的理解至关重要。这篇综述强调了此类研究的重要性，并涵盖了目前的EM成像（包括cryo-ET和相关混合技术）在PMHB上的应用中的挑战，局限性和最新发展。