Despite the significant medical advances which have taken place in the last decades, acute diarrhoea cases remain a public health issue of major significance, with gastroenteritis agents being associated with severe symptoms in adults and high morbidity in infants and children. Regarding rotaviruses, while children are the predominant victims of rotavirus infection, adults (often caretakers or parents of these children) may experience the same symptoms of fever, vomiting, and non-bloody diarrhoea. Three different routine schemes for the detection of rotaviruses in archived stool samples were evaluated in terms of diagnostic performance. A total of 640 archived stool samples were included in the study. The samples were screened with three different techniques: a commercial rapid immunochromatographic test, a modified in-house conventional one-step reverse-transcription polymerase chain reaction (PCR) screen protocol, and a com-mer-cial one-step real-time PCR kit. Technical aspects and considerations are discussed.

中文翻译：

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免疫色谱技术在轮状病毒检测中的应用：希腊中部三级医院的经验。

尽管在过去的几十年中医学取得了长足的进步，但是急性腹泻仍然是一个具有重大意义的公共卫生问题，胃肠炎病原体与成年人的严重症状以及婴儿和儿童的高发病率有关。关于轮状病毒，虽然儿童是轮状病毒感染的主要受害者，但成年人（通常是这些儿童的看护者或父母）可能会出现发烧，呕吐和非血性腹泻的相同症状。根据诊断性能评估了三种不同的检测粪便样本中轮状病毒的常规方案。该研究总共包括了640个存档的粪便样本。使用三种不同的技术筛选样品：商业快速免疫色谱测试，改进的内部常规一步一步逆转录聚合酶链反应（PCR）筛选方案，以及商业一步实时PCR试剂盒。讨论了技术方面和注意事项。