Ovarian torsion is agynecologic emergency that affects females of all ages. Early diagnosis is important to preserve ovarian function. The false positive rate for sonographic diagnosis of ovarian torsion is 50%; therefore, a new real-time approach is required to improve diagnostic accuracy. We investigated diffuse reflectance spectroscopy for diagnosing ovarian torsion. Spectroscopic measurements were performed in vivo prior to, during and after detorsion. After bilateral oophorectomy, hemoxygenase and myeloperoxidase enzyme activity in ovarian tissue was evaluated and the tissues were examined for pathology. Spectroscopic data were compared to histopathological and biochemical data to assess the diagnostic value of the spectroscopic method for differentiating healthy and damaged ovarian tissue. We found a good correlation between spectroscopy and histopathology. We also found a correlation between the spectroscopic data and heme oxygenase enzyme activity. We found no correlation between the histopathological tissue damage score and myeloperoxidase enzyme activity. Diffuse reflectance spectroscopy may be of prognostic and diagnostic value for ovarian torsion in vivo.

中文翻译：

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一种新型的卵巢扭转诊断方法的组织病理学和生化评估。

卵巢扭转是一种妇科急症，影响各个年龄段的女性。早期诊断对于保持卵巢功能很重要。超声检查诊断卵巢扭转的假阳性率为50％；因此，需要一种新的实时方法来提高诊断准确性。我们研究了漫反射光谱法用于诊断卵巢扭转。在扭曲之前，期间和之后在体内进行光谱测量。双侧卵巢切除术后，评估卵巢组织中的血氧合酶和髓过氧化物酶活性，并检查组织的病理学。将光谱数据与组织病理学和生化数据进行比较，以评估光谱方法对区分健康和受损卵巢组织的诊断价值。我们发现光谱学和组织病理学之间有良好的相关性。我们还发现光谱数据与血红素加氧酶活性之间存在相关性。我们发现组织病理学组织损伤评分与髓过氧化物酶活性之间无相关性。漫反射光谱法对体内卵巢扭转可能具有预后和诊断价值。